The mechanisms involved in the association between membrane movements and the initiation of DNA synthesis were approached measuring the synthesis of membrane-associated substances after stimulation of resting phase cultures. The inducement of the division cycle by fresh serum, FGF and a growth factor produced during RSV infection, caused a decreased incorporation of glucosamine and SO4 into focal adhesion sites. The decreased incorporation of glucosamine occurred during the G1 period, remained at a plateau during S and increased back to initial levels when DNA synthesis declined; it was inversely related to the size of the fraction of cells that entered the division cycle.
ASJC Scopus subject areas
- Cell Biology