Metabolic modulation induced by chronic hypoxia in rats using a comparative proteomic analysis of skeletal muscle tissue

S. De Palma, M. Ripamonti, A. Viganò, M. Moriggi, D. Capitanio, M. Samaja, G. Milano, P. Cerretelli, R. Wait, C. Gelfi

Research output: Contribution to journalArticlepeer-review

Abstract

Hypoxia-induced changes of rat skeletal muscle were investigated by two-dimensional difference ingel electrophoresis (2D-DIGE) and mass spectrometry. The results indicated that proteins involved in the TCA cycle, ATP production, and electron transport are down-regulated, whereas glycolytic enzymes and deaminases involved in ATP and AMP production were up-regulated. Up-regulation of the hypoxia markers hypoxia inducible factor 1 (HIF-1α) and pyruvate dehydrogenase kinase 1 (PDK1) was also observed, suggesting that in vivo adaptation to hypoxia requires an active metabolic switch. The kinase protein, mammalian target of rapamycin (mTOR), which has been implicated in the regulation of protein synthesis in hypoxia, appears unchanged, suggesting that its activity, in this system, is not controlled by oxygen partial pressure.

Original languageEnglish
Pages (from-to)1974-1984
Number of pages11
JournalJournal of Proteome Research
Volume6
Issue number5
DOIs
Publication statusPublished - May 2007

Keywords

  • 2D-DIGE
  • Hypoxia
  • Mass spectrometry
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics

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