Metabolism of arachidonic acid in isolated glomeruli from pig kidney

Manuela Livio, Chiara Chiabrando, Daniela Macconi, Ariela Benigni, Marcello Zimei, Maria Teresa De Pietro, Giuseppe Remuzzi

Research output: Contribution to journalArticle

Abstract

Arachidonic acid metabolism in isolated glomeruli from pig kidney was investigated. Arachidonic acid metabolism via cyclooxygenase was studied by three different methodological approaches: radioimmunoassay (RIA), high-performance liquid chromatography (HPLC) and gas Chromatograph-mass spectrometry (GC-MS). By all these techniques, the major prostaglandins (PG) formed by pig glomeruli appeared to be 6-keto-PGF and PGF, the former being the most abundant. RIA and GC-MS also detected lower amounts of thromboxane B2 (TxB2) and PGE2. This emphasises the similarity with human glomeruli, in which the main cyclooxygenase product has indeed been reported to be 6-keto-PGF. The lipoxygenase activity in isolated pig glomeruli, as studied by HPLC, generated 15-HETE, 12-HETE and 5-HETE. These data demonstrate that isolated glomeruli from pig kidney possess cyclooxygenase as well as lipoxygenase activity. Since a marked functional similarity exists between human and pig kidney, the pig can be regarded as a good model for studying the influence of arachidonic acid metabolites on glomerular pathophysiology.

Original languageEnglish
Pages (from-to)110-121
Number of pages12
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume961
Issue number1
DOIs
Publication statusPublished - Jul 1 1988

Keywords

  • (Pig kidney glomeruli)
  • Arachidonic acid metabolism
  • Lipoxygenase activity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

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