Metabolism of arachidonic acid in isolated glomeruli from pig kidney

Manuela Livio, Chiara Chiabrando, Daniela Macconi, Ariela Benigni, Marcello Zimei, Maria Teresa De Pietro, Giuseppe Remuzzi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Arachidonic acid metabolism in isolated glomeruli from pig kidney was investigated. Arachidonic acid metabolism via cyclooxygenase was studied by three different methodological approaches: radioimmunoassay (RIA), high-performance liquid chromatography (HPLC) and gas Chromatograph-mass spectrometry (GC-MS). By all these techniques, the major prostaglandins (PG) formed by pig glomeruli appeared to be 6-keto-PGF and PGF, the former being the most abundant. RIA and GC-MS also detected lower amounts of thromboxane B2 (TxB2) and PGE2. This emphasises the similarity with human glomeruli, in which the main cyclooxygenase product has indeed been reported to be 6-keto-PGF. The lipoxygenase activity in isolated pig glomeruli, as studied by HPLC, generated 15-HETE, 12-HETE and 5-HETE. These data demonstrate that isolated glomeruli from pig kidney possess cyclooxygenase as well as lipoxygenase activity. Since a marked functional similarity exists between human and pig kidney, the pig can be regarded as a good model for studying the influence of arachidonic acid metabolites on glomerular pathophysiology.

Original languageEnglish
Pages (from-to)110-121
Number of pages12
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume961
Issue number1
DOIs
Publication statusPublished - Jul 1 1988

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Kidney Glomerulus
Prostaglandin-Endoperoxide Synthases
Arachidonic Acid
Metabolism
Swine
Lipoxygenase
High performance liquid chromatography
Mass spectrometry
Gases
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Thromboxane B2
Dinoprost
Metabolites
Dinoprostone
Prostaglandins
Radioimmunoassay
Mass Spectrometry
High Pressure Liquid Chromatography
Kidney
prostaglandin F1

Keywords

  • (Pig kidney glomeruli)
  • Arachidonic acid metabolism
  • Lipoxygenase activity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Metabolism of arachidonic acid in isolated glomeruli from pig kidney. / Livio, Manuela; Chiabrando, Chiara; Macconi, Daniela; Benigni, Ariela; Zimei, Marcello; De Pietro, Maria Teresa; Remuzzi, Giuseppe.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 961, No. 1, 01.07.1988, p. 110-121.

Research output: Contribution to journalArticle

Livio, Manuela ; Chiabrando, Chiara ; Macconi, Daniela ; Benigni, Ariela ; Zimei, Marcello ; De Pietro, Maria Teresa ; Remuzzi, Giuseppe. / Metabolism of arachidonic acid in isolated glomeruli from pig kidney. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1988 ; Vol. 961, No. 1. pp. 110-121.
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