TY - JOUR
T1 - Metabolism of exogenous ganglioside GM1 in cultured cerebellar granule cells The fatty acid and sphingosine moieties formed during degradation are re-used for lipid biosynthesis
AU - Riboni, Laura
AU - Bassi, Rosaria
AU - Conti, Mauro
AU - Tettamanti, Guido
PY - 1993/5/17
Y1 - 1993/5/17
N2 - Cerebellar granule cells, differentiated in vitro, were parallelly fed with [Sph-3H]GM1 and [stearoyl-14C]GM1, under identical conditions (10-6 M ganglioside; pulse, from 1-4 h; chase, up to 24 h after 4 h pulse) and the salvage pathways of sphingosine and stearic acid were investigated. It was observed that both sphingosine and stearic acid, liberated during the intralysosomal degradation of ganglioside, are metabolically recycled, along distinct pathways. Sphingosine is used for the biosynthesis of a number of sphingolipids, particularly ceramide, glucosyl-ceramide, gangliosides and sphingomyelin; stearic acid is utilized for the biosynthesis of sphingolipids, and to a greater extent, glycero-phospholipids, especially those endogenously richer in stearic acid (phosphatidyl-ethanolamine and phosphatidyl-choline). No evidence was provided for a salvage pathway for ceramide.
AB - Cerebellar granule cells, differentiated in vitro, were parallelly fed with [Sph-3H]GM1 and [stearoyl-14C]GM1, under identical conditions (10-6 M ganglioside; pulse, from 1-4 h; chase, up to 24 h after 4 h pulse) and the salvage pathways of sphingosine and stearic acid were investigated. It was observed that both sphingosine and stearic acid, liberated during the intralysosomal degradation of ganglioside, are metabolically recycled, along distinct pathways. Sphingosine is used for the biosynthesis of a number of sphingolipids, particularly ceramide, glucosyl-ceramide, gangliosides and sphingomyelin; stearic acid is utilized for the biosynthesis of sphingolipids, and to a greater extent, glycero-phospholipids, especially those endogenously richer in stearic acid (phosphatidyl-ethanolamine and phosphatidyl-choline). No evidence was provided for a salvage pathway for ceramide.
KW - Cerebellar granule cell
KW - Fatty acid
KW - Ganglioside
KW - Metabolic salvage pathway
KW - Phospholipid
KW - Sphingosine
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U2 - 10.1016/0014-5793(93)81582-K
DO - 10.1016/0014-5793(93)81582-K
M3 - Article
C2 - 8486159
AN - SCOPUS:0027154901
VL - 322
SP - 257
EP - 260
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -