Metabolism of thromboxane B2 in the isolated perfused rat kidney: mass spectrometric identification of urinary products

Chiara Chiabrando, Vittorio Pinciroli, Norberto Perico, Andrea Campoleoni, Ariela Benigni, Giuseppe Remuzzi, Roberto Fanelli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The metabolism of thromboxane B2 (TXB2), the stable breakdown product of thromboxane A2, has been studied in isolated perfused kidney preparations using a recirculating system. In a first experiment, TXB2 was infused at a rate of 20 μg/kg per min. In a second experiment, a 1:1 mixture of TXB2 and octadeuterated TXB2 (0.4 μg/kg per min each) was infused. Urinary samples collected during the infusion of TXB2 or vehicle were extracted on C18 cartridges and derivatized to methyl or pentafluorobenzyl ester, methyloxime, trimethylsilyl ether. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the electron impact and negative ion chemical ionization modes. Products of β-oxidation, reduction of the Δ5,6 double bond and dehydrogenation at C-11 (2,3-dinor-TXB2, 2,3-dinor-TXB1, 2,3,4,5-tetranor-TXB1 and 11-dehydro-TXB2) were identified in addition to unmetabolized TXB2. 2,3,4,5-tetranor-TXB1 and 2, 3, dinor-TXB1 were the most abundant metabolites.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1006
Issue number2
DOIs
Publication statusPublished - Nov 28 1989

Fingerprint

Thromboxane B2
Metabolism
Rats
Kidney
Thromboxane A2
Dehydrogenation
Metabolites
Gas chromatography
Ether
Gas Chromatography-Mass Spectrometry
Oxidation-Reduction
Ionization
Mass spectrometry
Esters
Negative ions
Experiments
Electrons
Ions

Keywords

  • (Rat kidney)
  • GC-MS
  • Thromboxane metabolism

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

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title = "Metabolism of thromboxane B2 in the isolated perfused rat kidney: mass spectrometric identification of urinary products",
abstract = "The metabolism of thromboxane B2 (TXB2), the stable breakdown product of thromboxane A2, has been studied in isolated perfused kidney preparations using a recirculating system. In a first experiment, TXB2 was infused at a rate of 20 μg/kg per min. In a second experiment, a 1:1 mixture of TXB2 and octadeuterated TXB2 (0.4 μg/kg per min each) was infused. Urinary samples collected during the infusion of TXB2 or vehicle were extracted on C18 cartridges and derivatized to methyl or pentafluorobenzyl ester, methyloxime, trimethylsilyl ether. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the electron impact and negative ion chemical ionization modes. Products of β-oxidation, reduction of the Δ5,6 double bond and dehydrogenation at C-11 (2,3-dinor-TXB2, 2,3-dinor-TXB1, 2,3,4,5-tetranor-TXB1 and 11-dehydro-TXB2) were identified in addition to unmetabolized TXB2. 2,3,4,5-tetranor-TXB1 and 2, 3, dinor-TXB1 were the most abundant metabolites.",
keywords = "(Rat kidney), GC-MS, Thromboxane metabolism",
author = "Chiara Chiabrando and Vittorio Pinciroli and Norberto Perico and Andrea Campoleoni and Ariela Benigni and Giuseppe Remuzzi and Roberto Fanelli",
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T2 - mass spectrometric identification of urinary products

AU - Chiabrando, Chiara

AU - Pinciroli, Vittorio

AU - Perico, Norberto

AU - Campoleoni, Andrea

AU - Benigni, Ariela

AU - Remuzzi, Giuseppe

AU - Fanelli, Roberto

PY - 1989/11/28

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N2 - The metabolism of thromboxane B2 (TXB2), the stable breakdown product of thromboxane A2, has been studied in isolated perfused kidney preparations using a recirculating system. In a first experiment, TXB2 was infused at a rate of 20 μg/kg per min. In a second experiment, a 1:1 mixture of TXB2 and octadeuterated TXB2 (0.4 μg/kg per min each) was infused. Urinary samples collected during the infusion of TXB2 or vehicle were extracted on C18 cartridges and derivatized to methyl or pentafluorobenzyl ester, methyloxime, trimethylsilyl ether. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the electron impact and negative ion chemical ionization modes. Products of β-oxidation, reduction of the Δ5,6 double bond and dehydrogenation at C-11 (2,3-dinor-TXB2, 2,3-dinor-TXB1, 2,3,4,5-tetranor-TXB1 and 11-dehydro-TXB2) were identified in addition to unmetabolized TXB2. 2,3,4,5-tetranor-TXB1 and 2, 3, dinor-TXB1 were the most abundant metabolites.

AB - The metabolism of thromboxane B2 (TXB2), the stable breakdown product of thromboxane A2, has been studied in isolated perfused kidney preparations using a recirculating system. In a first experiment, TXB2 was infused at a rate of 20 μg/kg per min. In a second experiment, a 1:1 mixture of TXB2 and octadeuterated TXB2 (0.4 μg/kg per min each) was infused. Urinary samples collected during the infusion of TXB2 or vehicle were extracted on C18 cartridges and derivatized to methyl or pentafluorobenzyl ester, methyloxime, trimethylsilyl ether. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the electron impact and negative ion chemical ionization modes. Products of β-oxidation, reduction of the Δ5,6 double bond and dehydrogenation at C-11 (2,3-dinor-TXB2, 2,3-dinor-TXB1, 2,3,4,5-tetranor-TXB1 and 11-dehydro-TXB2) were identified in addition to unmetabolized TXB2. 2,3,4,5-tetranor-TXB1 and 2, 3, dinor-TXB1 were the most abundant metabolites.

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