Metabolomic profile of amniotic fluid to evaluate lung maturity: The diaphragmatic hernia lamb model

Gloria Pelizzo, Maurizio Ballico, Maria Chiara Mimmi, José Louis Peirò, Mario Marotta, Costanzo Federico, Erika Andreatta, Ghassan Nakib, Maurilio Sampaolesi, Elisa Zambaiti, Valeria Calcaterra

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Tracheal occlusion (TO) stimulates lung growth in fetuses affected with congenital diaphragmatic hernia (CDH) although the processes involved in lung maturation still remain unknown. The objective of this study was to evaluate the metabolomic profile of amniotic fluid (AF) following TO in fetal lamb model in order to obtain an indirect view of mechanisms involved in pulmonary reversal hypoplasia and biochemical maturity in response to fetal TO. Methods: Liquid Chromatography Mass Spectrometry was performed on lamb AF samples at: age I (70 days' gestation); age II (102 days' gestation); age III (136 days' gestation). CDH was induced at age I and TO at age II. Results: Betaine, choline, creatinine were found significantly increased during gestation in the control group. The CDH group showed choline (p =0.007) and creatinine (p =0.004) decreases during pregnancy. In the TO group choline and creatinine profiles were restored. Conclusions: Alveolar tissue and fetal global growth ameliorated after TO. Metabolomics provided usefu information on biochemical details during lung maturation. Metabolomic profiling would help to identify the best time to perform TO, in order to increase survival of CDH affected patients.

Original languageEnglish
Article number54
JournalMultidisciplinary Respiratory Medicine
Volume9
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Amniotic fluid
  • Animal model
  • Congenital diaphragmatic hernia
  • Metabolomic
  • Tracheal occlusion

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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