Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development

C. Agresti, M. E. Meomartini, S. Amadio, E. Ambrosini, B. Serafini, L. Franchini, C. Volonté, F. Aloisi, S. Visentin

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

To gain insights into the role of purinergic receptors in oligodendrocyte development, we characterized the expression and functional activity of P2 receptors in cultured rat oligodendrocyte progenitors and investigated the effects of ATP and its breakdown products on the migration and proliferation of this immature glial cell population. Using Western blot analysis, we show that oligodendrocyte progenitors express several P2X (P2X1,2,3,4,7) and P2Y (P2Y1,2,4) receptors. Intracellular Ca2+ recording by Fura-2 video imaging allowed to determine the rank potency order of the P2 agonists tested: ADPβS = ADP = Benzoyl ATP > ATP > ATPγS > UTP, α,β-meATP ineffective. Based on the above findings, on pharmacological inhibition by the antagonists oxATP and MRS2179, and on the absence of α,βmeATP-induced inward current in whole-cell recording, P2X7 and P2Y1 were identified as the main ionotropic and metabotropic P2 receptors active in OPs. As a functional correlate of these findings, we show that ATP and, among metabotropic agonists, ADP and the P2Y1-specific agonist ADPβS, but not UTP, induce oligodendrocyte progenitor migration. Moreover, ATP and ADP inhibited the proliferation of oligodendrocyte progenitors induced by platelet-derived growth factor, both in purified cultures and in cerebellar tissue slices. The effects of ATP and ADP on cell migration and proliferation were prevented by the P2Y1 antagonist MRS2179. By confocal laser scanning microscopy, P2Y1 receptors were localized in NG2-labeled oligodendrocyte progenitors in the developing rat brain. These data indicate that ATP and ADP may regulate oligodendrocyte progenitor functions by a mechanism that involves mainly activation of P2Y1 receptors.

Original languageEnglish
Pages (from-to)132-144
Number of pages13
JournalGLIA
Volume50
Issue number2
DOIs
Publication statusPublished - Apr 15 2005

Fingerprint

Oligodendroglia
Adenosine Triphosphate
Adenosine Diphosphate
Purinergic P2Y1 Receptors
Uridine Triphosphate
Purinergic Receptors
Fura-2
Platelet-Derived Growth Factor
Patch-Clamp Techniques
Confocal Microscopy
Neuroglia
Cell Movement
Western Blotting
Cell Proliferation
Pharmacology
Brain
Population

Keywords

  • ATP
  • Cell motility
  • Intracellular calcium
  • P2X
  • P2Y
  • Remyelination

ASJC Scopus subject areas

  • Immunology

Cite this

Agresti, C., Meomartini, M. E., Amadio, S., Ambrosini, E., Serafini, B., Franchini, L., ... Visentin, S. (2005). Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development. GLIA, 50(2), 132-144. https://doi.org/10.1002/glia.20160

Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development. / Agresti, C.; Meomartini, M. E.; Amadio, S.; Ambrosini, E.; Serafini, B.; Franchini, L.; Volonté, C.; Aloisi, F.; Visentin, S.

In: GLIA, Vol. 50, No. 2, 15.04.2005, p. 132-144.

Research output: Contribution to journalArticle

Agresti, C, Meomartini, ME, Amadio, S, Ambrosini, E, Serafini, B, Franchini, L, Volonté, C, Aloisi, F & Visentin, S 2005, 'Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development', GLIA, vol. 50, no. 2, pp. 132-144. https://doi.org/10.1002/glia.20160
Agresti C, Meomartini ME, Amadio S, Ambrosini E, Serafini B, Franchini L et al. Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development. GLIA. 2005 Apr 15;50(2):132-144. https://doi.org/10.1002/glia.20160
Agresti, C. ; Meomartini, M. E. ; Amadio, S. ; Ambrosini, E. ; Serafini, B. ; Franchini, L. ; Volonté, C. ; Aloisi, F. ; Visentin, S. / Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development. In: GLIA. 2005 ; Vol. 50, No. 2. pp. 132-144.
@article{2cc24f9f06874f46b585e9870b03d04a,
title = "Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development",
abstract = "To gain insights into the role of purinergic receptors in oligodendrocyte development, we characterized the expression and functional activity of P2 receptors in cultured rat oligodendrocyte progenitors and investigated the effects of ATP and its breakdown products on the migration and proliferation of this immature glial cell population. Using Western blot analysis, we show that oligodendrocyte progenitors express several P2X (P2X1,2,3,4,7) and P2Y (P2Y1,2,4) receptors. Intracellular Ca2+ recording by Fura-2 video imaging allowed to determine the rank potency order of the P2 agonists tested: ADPβS = ADP = Benzoyl ATP > ATP > ATPγS > UTP, α,β-meATP ineffective. Based on the above findings, on pharmacological inhibition by the antagonists oxATP and MRS2179, and on the absence of α,βmeATP-induced inward current in whole-cell recording, P2X7 and P2Y1 were identified as the main ionotropic and metabotropic P2 receptors active in OPs. As a functional correlate of these findings, we show that ATP and, among metabotropic agonists, ADP and the P2Y1-specific agonist ADPβS, but not UTP, induce oligodendrocyte progenitor migration. Moreover, ATP and ADP inhibited the proliferation of oligodendrocyte progenitors induced by platelet-derived growth factor, both in purified cultures and in cerebellar tissue slices. The effects of ATP and ADP on cell migration and proliferation were prevented by the P2Y1 antagonist MRS2179. By confocal laser scanning microscopy, P2Y1 receptors were localized in NG2-labeled oligodendrocyte progenitors in the developing rat brain. These data indicate that ATP and ADP may regulate oligodendrocyte progenitor functions by a mechanism that involves mainly activation of P2Y1 receptors.",
keywords = "ATP, Cell motility, Intracellular calcium, P2X, P2Y, Remyelination",
author = "C. Agresti and Meomartini, {M. E.} and S. Amadio and E. Ambrosini and B. Serafini and L. Franchini and C. Volont{\'e} and F. Aloisi and S. Visentin",
year = "2005",
month = "4",
day = "15",
doi = "10.1002/glia.20160",
language = "English",
volume = "50",
pages = "132--144",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development

AU - Agresti, C.

AU - Meomartini, M. E.

AU - Amadio, S.

AU - Ambrosini, E.

AU - Serafini, B.

AU - Franchini, L.

AU - Volonté, C.

AU - Aloisi, F.

AU - Visentin, S.

PY - 2005/4/15

Y1 - 2005/4/15

N2 - To gain insights into the role of purinergic receptors in oligodendrocyte development, we characterized the expression and functional activity of P2 receptors in cultured rat oligodendrocyte progenitors and investigated the effects of ATP and its breakdown products on the migration and proliferation of this immature glial cell population. Using Western blot analysis, we show that oligodendrocyte progenitors express several P2X (P2X1,2,3,4,7) and P2Y (P2Y1,2,4) receptors. Intracellular Ca2+ recording by Fura-2 video imaging allowed to determine the rank potency order of the P2 agonists tested: ADPβS = ADP = Benzoyl ATP > ATP > ATPγS > UTP, α,β-meATP ineffective. Based on the above findings, on pharmacological inhibition by the antagonists oxATP and MRS2179, and on the absence of α,βmeATP-induced inward current in whole-cell recording, P2X7 and P2Y1 were identified as the main ionotropic and metabotropic P2 receptors active in OPs. As a functional correlate of these findings, we show that ATP and, among metabotropic agonists, ADP and the P2Y1-specific agonist ADPβS, but not UTP, induce oligodendrocyte progenitor migration. Moreover, ATP and ADP inhibited the proliferation of oligodendrocyte progenitors induced by platelet-derived growth factor, both in purified cultures and in cerebellar tissue slices. The effects of ATP and ADP on cell migration and proliferation were prevented by the P2Y1 antagonist MRS2179. By confocal laser scanning microscopy, P2Y1 receptors were localized in NG2-labeled oligodendrocyte progenitors in the developing rat brain. These data indicate that ATP and ADP may regulate oligodendrocyte progenitor functions by a mechanism that involves mainly activation of P2Y1 receptors.

AB - To gain insights into the role of purinergic receptors in oligodendrocyte development, we characterized the expression and functional activity of P2 receptors in cultured rat oligodendrocyte progenitors and investigated the effects of ATP and its breakdown products on the migration and proliferation of this immature glial cell population. Using Western blot analysis, we show that oligodendrocyte progenitors express several P2X (P2X1,2,3,4,7) and P2Y (P2Y1,2,4) receptors. Intracellular Ca2+ recording by Fura-2 video imaging allowed to determine the rank potency order of the P2 agonists tested: ADPβS = ADP = Benzoyl ATP > ATP > ATPγS > UTP, α,β-meATP ineffective. Based on the above findings, on pharmacological inhibition by the antagonists oxATP and MRS2179, and on the absence of α,βmeATP-induced inward current in whole-cell recording, P2X7 and P2Y1 were identified as the main ionotropic and metabotropic P2 receptors active in OPs. As a functional correlate of these findings, we show that ATP and, among metabotropic agonists, ADP and the P2Y1-specific agonist ADPβS, but not UTP, induce oligodendrocyte progenitor migration. Moreover, ATP and ADP inhibited the proliferation of oligodendrocyte progenitors induced by platelet-derived growth factor, both in purified cultures and in cerebellar tissue slices. The effects of ATP and ADP on cell migration and proliferation were prevented by the P2Y1 antagonist MRS2179. By confocal laser scanning microscopy, P2Y1 receptors were localized in NG2-labeled oligodendrocyte progenitors in the developing rat brain. These data indicate that ATP and ADP may regulate oligodendrocyte progenitor functions by a mechanism that involves mainly activation of P2Y1 receptors.

KW - ATP

KW - Cell motility

KW - Intracellular calcium

KW - P2X

KW - P2Y

KW - Remyelination

UR - http://www.scopus.com/inward/record.url?scp=18244367900&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18244367900&partnerID=8YFLogxK

U2 - 10.1002/glia.20160

DO - 10.1002/glia.20160

M3 - Article

VL - 50

SP - 132

EP - 144

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 2

ER -