Metal-score as a potential non-invasive diagnostic test for Alzheimer's disease

Rosanna Squitti, Patrizio Pasqualetti, Renato Polimanti, Carlo Salustri, Filomena Moffa, Emanuele Cassetta, Domenico Lupoi, Mariacarla Ventriglia, Maurizio Cortesi, Mariacristina Siotto, Fabrizio Vernieri, Paolo Maria Rossini

Research output: Contribution to journalArticle

Abstract

The link between biometals and Alzheimer's disease (AD) has been investigated with a focus on local metal accumulations. In this work, we have looked at systemic metal changes and computed a score (M-score) based on metal disarrangements to discriminate patients with AD from patients with vascular dementia (VaD) and from controls. We measured serum levels of iron, copper, ceruloplasmin, transferrin, and total antioxidant capacity (TAS), performed Apolipoprotein E (APOE) genotyping and calculated non-ceruloplasmin copper ('free' copper') levels, transferrin saturation, total iron binding capacity, and ceruloplasmin-transferrin ratio (Cp/Tf) in 93 patients with AD, 45 patients with VaD, and 48 controls. All subjects underwent biochemical, neuroimaging and cognitive evaluations. Significant differences were observed among the tested groups for the levels of copper, free copper, peroxides, and TAS and for the Cp/Tf with disparity in couple comparison. On this basis we created the M-score as linear combination of biometal variables and APOE genotype. Besides its ability to discriminate AD patients vs. controls (ROC AUC=90%), M-score was able to distinguish AD vs. VaD (ROC AUC=79%). Moreover, we calculated the sensitivity and the specificity for M-score and for the other significant variables: M-score reached the highest sensitivity without a relevant loss in terms of specificity. When we compared M-score with APOE genotype and Medial Temporal Atrophy score, it resulted statistically better than these diagnostic markers. In conclusion, we confirm the link between biometals and AD and suggest its potential as diagnostic tool. Further studies may elucidate its potential role as reliable diagnostic test.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalCurrent Alzheimer Research
Volume10
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Routine Diagnostic Tests
Alzheimer Disease
Metals
Copper
Transferrin
Vascular Dementia
Ceruloplasmin
Trace Elements
Apolipoproteins E
Area Under Curve
Iron
Genotype
Aptitude
Peroxides
Neuroimaging
Atrophy
Antioxidants
Sensitivity and Specificity
Serum

Keywords

  • Alzheimer's disease
  • Antioxidant capacity
  • Copper
  • Diagnostic test
  • Metal disarrangements
  • Statistical score
  • Transferrin
  • Vascular dementia

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Metal-score as a potential non-invasive diagnostic test for Alzheimer's disease. / Squitti, Rosanna; Pasqualetti, Patrizio; Polimanti, Renato; Salustri, Carlo; Moffa, Filomena; Cassetta, Emanuele; Lupoi, Domenico; Ventriglia, Mariacarla; Cortesi, Maurizio; Siotto, Mariacristina; Vernieri, Fabrizio; Rossini, Paolo Maria.

In: Current Alzheimer Research, Vol. 10, No. 2, 2013, p. 191-198.

Research output: Contribution to journalArticle

Squitti, R, Pasqualetti, P, Polimanti, R, Salustri, C, Moffa, F, Cassetta, E, Lupoi, D, Ventriglia, M, Cortesi, M, Siotto, M, Vernieri, F & Rossini, PM 2013, 'Metal-score as a potential non-invasive diagnostic test for Alzheimer's disease', Current Alzheimer Research, vol. 10, no. 2, pp. 191-198. https://doi.org/10.2174/1567205011310020009
Squitti R, Pasqualetti P, Polimanti R, Salustri C, Moffa F, Cassetta E et al. Metal-score as a potential non-invasive diagnostic test for Alzheimer's disease. Current Alzheimer Research. 2013;10(2):191-198. https://doi.org/10.2174/1567205011310020009
Squitti, Rosanna ; Pasqualetti, Patrizio ; Polimanti, Renato ; Salustri, Carlo ; Moffa, Filomena ; Cassetta, Emanuele ; Lupoi, Domenico ; Ventriglia, Mariacarla ; Cortesi, Maurizio ; Siotto, Mariacristina ; Vernieri, Fabrizio ; Rossini, Paolo Maria. / Metal-score as a potential non-invasive diagnostic test for Alzheimer's disease. In: Current Alzheimer Research. 2013 ; Vol. 10, No. 2. pp. 191-198.
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AU - Cassetta, Emanuele

AU - Lupoi, Domenico

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