Metallo-β-lactamase producers in environmental microbiota

New molecular class B enzyme in Janthinobacterium lividum

G. M. Rossolini, M. A. Condemi, F. Pantanella, J. D. Docquier, G. Amicosante, M. C. Thaller

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Eleven environmental samples from different sources were screened for the presence of metallo-β-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of EDTA-inhibitable carbapenemase activity. A total of 15 metallo-β-lactamase-producing isolates, including 10 Stenotrophomonas maltophilia isolates, 3 Chryseobacterium spp., one Aeromonas hydrophila isolate, and one Janthinobacterium lividum isolate (a species in which production of metallo-β-lactamase activity was not previously reported), were obtained from 8 samples. In the J. lividum isolate, named JAC1, production of metallo-β-lactamase activity was elicited upon exposure to β-lactams. Screening of a JAC1 genomic library for clones showing a reduced imipenem susceptibility led to the isolation of a metallo-β-lactamase determinant encoding a new member (named THIN-B) of the highly divergent subclass B3 lineage of metallo-β-lactamases. THIN-B is most closely related (35.6% identical residues) to the L1 enzyme of S. maltophilia and more distantly related to the FEZ-1 enzyme of Legionella gormanii (27.8% identity) and to the GOB-1 enzyme of Chryseobacterium meningosepticum (24.2% identity). Sequences related to blaTHIN-B, and inducible production of metallo-β-lactamase activity, were also detected in the J. lividum type strain DSM1522. Expression of the blaTHIN-B gene in Escherichia coli resulted in decreased susceptibility to several β-lactams, including penicillins, cephalosporins (including cephamycins and oxyimino cephalosporins), and carbapenems, revealing a broad substrate specificity of the enzyme. The results of this study indicated that metallo-β-lactamase-producing bacteria are widespread in the environment and identified a new molecular class B enzyme in the environmental species J. lividum.

Original languageEnglish
Pages (from-to)837-844
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume45
Issue number3
DOIs
Publication statusPublished - 2001

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Microbiota
Chryseobacterium
Stenotrophomonas maltophilia
Enzymes
Lactams
Carbapenems
Cephalosporins
Cephamycins
Bacteria
Aeromonas hydrophila
Legionella
Genomic Library
Imipenem
Substrate Specificity
Edetic Acid
Penicillins
Clone Cells
Escherichia coli
Genes

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Metallo-β-lactamase producers in environmental microbiota : New molecular class B enzyme in Janthinobacterium lividum. / Rossolini, G. M.; Condemi, M. A.; Pantanella, F.; Docquier, J. D.; Amicosante, G.; Thaller, M. C.

In: Antimicrobial Agents and Chemotherapy, Vol. 45, No. 3, 2001, p. 837-844.

Research output: Contribution to journalArticle

Rossolini, G. M. ; Condemi, M. A. ; Pantanella, F. ; Docquier, J. D. ; Amicosante, G. ; Thaller, M. C. / Metallo-β-lactamase producers in environmental microbiota : New molecular class B enzyme in Janthinobacterium lividum. In: Antimicrobial Agents and Chemotherapy. 2001 ; Vol. 45, No. 3. pp. 837-844.
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