Metastatic ability and differentiative properties of a new cell line of human embryonal rhabdomyosarcoma (CCA)

C. De Giovanni, P. Nanni, G. Nicoletti, C. Ceccarelli, K. Scotlandi, L. Landuzzi, P. L. Lollini

Research output: Contribution to journalArticle

Abstract

A new cell line (CCA) was established from a human embryonal rhabdomyosarcoma. It showed an 'early' myogenic differentiation pattern: vimentin expression was found in 100% of cells, desmin in about 40% and myosin of the embryonic isoform in about 5%. Class I HLA expression on CCA cells was undetectable but was greatly increased by in vitro treatment with human recombinant interferon-gamma and only marginally increased by human recombinant tumour necrosis factor-alfa. CCA cell line was tumorigenic in nude mice after either subcutaneous or intramuscular injection; macroscopic spontaneous metastases were not detected. The ability to induce metastatic nodules in the lungs was found when CCA cells were injected intravenously in cyclophosphamide-pretreated nude mice and, at low frequency, in untreated nude mice.

Original languageEnglish
Pages (from-to)1943-1950
Number of pages8
JournalAnticancer Research
Volume9
Issue number6
Publication statusPublished - 1989

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Keywords

  • human rhabdomyosarcoma
  • interferon-gamma
  • metastasis
  • myogenic differentiation
  • nude mice
  • tumor necrosis factor-alfa

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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