Metastatic behavior of an adriamycin-resistant murine tumor

R. Giavazzi, L. Miller, I. R. Hart

Research output: Contribution to journalArticle

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Abstract

The metastatic behavior of a murine fibrosarcoma (UV-2237M-ADM®) resistant to Adriamycin (ADM) (doxorubicin) was investigated. Subclones isolated from the UV-2237M-ADM® line, which originated from a single colony, generally displayed a similar degree of resistance to ADM (eight out of nine clones did not differ from this UV-2237M-ADM® line). In contrast, they differed significantly in their capacity to form lung colonies after i.v. injection (six of nine clones differed significantly from the UV-2237M-ADM® line, p ≤ 0.005, Mann-Whitney U test). The UV-2237M-ADM® cell line maintained resistance to ADM even after 17 weeks of growth in syngeneic mice, although a gradual decrease in resistance was observed over this time. Spontaneous metastases from the UV-2237M-ADM® tumor commonly retained resistance to ADM. Of 18 cell lines, each established from an individual lung nodule, 16 showed plating efficiencies in the presence of ADM comparable to that of the primary UV-2237M-ADM® tumor. The remaining two lines had partially reverted to the sensitive state. The i.v. administration of ADM significantly reduced the lung tumor burden of mice with ADM-sensitive UV-2237M tumors but failed to affect the lung tumor burden of mice with UV-2237M-ADM® tumors. The UV-2237M-ADM® tumor line, exhibiting as it does both drug-resistant and metastatic behavior, provides a useful model system with which to investigate the metastatic process and the development of drug resistance.

Original languageEnglish
Pages (from-to)5081-5086
Number of pages6
JournalCancer Research
Volume43
Issue number11
Publication statusPublished - 1983

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Doxorubicin
Neoplasms
Lung
Tumor Burden
Clone Cells
Cell Line
Fibrosarcoma
Nonparametric Statistics
Drug Resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Giavazzi, R., Miller, L., & Hart, I. R. (1983). Metastatic behavior of an adriamycin-resistant murine tumor. Cancer Research, 43(11), 5081-5086.

Metastatic behavior of an adriamycin-resistant murine tumor. / Giavazzi, R.; Miller, L.; Hart, I. R.

In: Cancer Research, Vol. 43, No. 11, 1983, p. 5081-5086.

Research output: Contribution to journalArticle

Giavazzi, R, Miller, L & Hart, IR 1983, 'Metastatic behavior of an adriamycin-resistant murine tumor', Cancer Research, vol. 43, no. 11, pp. 5081-5086.
Giavazzi, R. ; Miller, L. ; Hart, I. R. / Metastatic behavior of an adriamycin-resistant murine tumor. In: Cancer Research. 1983 ; Vol. 43, No. 11. pp. 5081-5086.
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title = "Metastatic behavior of an adriamycin-resistant murine tumor",
abstract = "The metastatic behavior of a murine fibrosarcoma (UV-2237M-ADM{\circledR}) resistant to Adriamycin (ADM) (doxorubicin) was investigated. Subclones isolated from the UV-2237M-ADM{\circledR} line, which originated from a single colony, generally displayed a similar degree of resistance to ADM (eight out of nine clones did not differ from this UV-2237M-ADM{\circledR} line). In contrast, they differed significantly in their capacity to form lung colonies after i.v. injection (six of nine clones differed significantly from the UV-2237M-ADM{\circledR} line, p ≤ 0.005, Mann-Whitney U test). The UV-2237M-ADM{\circledR} cell line maintained resistance to ADM even after 17 weeks of growth in syngeneic mice, although a gradual decrease in resistance was observed over this time. Spontaneous metastases from the UV-2237M-ADM{\circledR} tumor commonly retained resistance to ADM. Of 18 cell lines, each established from an individual lung nodule, 16 showed plating efficiencies in the presence of ADM comparable to that of the primary UV-2237M-ADM{\circledR} tumor. The remaining two lines had partially reverted to the sensitive state. The i.v. administration of ADM significantly reduced the lung tumor burden of mice with ADM-sensitive UV-2237M tumors but failed to affect the lung tumor burden of mice with UV-2237M-ADM{\circledR} tumors. The UV-2237M-ADM{\circledR} tumor line, exhibiting as it does both drug-resistant and metastatic behavior, provides a useful model system with which to investigate the metastatic process and the development of drug resistance.",
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