Methicillin-resistant Staphylococcus aureus in hospitalized patients from the Bolivian Chaco

Alessandro Bartoloni, Eleonora Riccobono, Donata Magnelli, Ana Liz Villagran, Tiziana Di Maggio, Antonia Mantella, Samanta Sennati, Carmen Revollo, Marianne Strohmeyer, Tommaso Giani, Lucia Pallecchi, Gian Maria Rossolini

Research output: Contribution to journalArticle

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Abstract

Objectives: Information is lacking on the methicillin-resistant Staphylococcus aureus (MRSA) clonal lineages circulating in Bolivia. We investigated the prevalence and molecular epidemiology of S. aureus colonization in hospitalized patients from the Bolivian Chaco, and compared their features with those of the few clinical isolates available from that setting. Methods: S. aureus nasal/inguinal colonization was investigated in 280 inpatients from eight hospitals in two point prevalence surveys (2012, n=. 90; 2013, n=. 190). Molecular characterization included genotyping (. spa typing, multilocus sequence typing, and pulsed-field gel electrophoresis), detection of virulence genes, and SCC. mec typing. Results: Forty-one inpatients (14.6%) were S. aureus nasal/inguinal carriers, of whom five were colonized by MRSA (1.8%). MRSA isolates mostly belonged to spa-type t701, harboured SCC. mec IVc, and were negative for Panton-Valentine leukocidin (PVL) genes. However, a USA300-related isolate was also detected, which showed the characteristics of the USA300 Latin American variant (USA300-LV; i.e., ST8, spa-type t008, SCC. mec IVc, presence of PVL genes, absence of arcA). Notably, all the available MRSA clinical isolates (. n=. 5, collected during 2011-2013) were also identified as USA300-LV. Conclusions: Overall, MRSA colonization in inpatients from the Bolivian Chaco was low. However, USA300-LV-related isolates were detected in colonization and infections, emphasizing the importance of implementing control measures to limit their further dissemination in this resource-limited area.

Original languageEnglish
Pages (from-to)e156-e160
JournalInternational Journal of Infectious Diseases
Volume30
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Inpatients
Groin
Nose
Genes
Bolivia
Multilocus Sequence Typing
Molecular Epidemiology
Pulsed Field Gel Electrophoresis
Virulence
Infection
Panton-Valentine leukocidin

Keywords

  • Bolivia
  • Carriage
  • Infection
  • Latin america
  • MRSA
  • USA300-LV

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

Cite this

Bartoloni, A., Riccobono, E., Magnelli, D., Villagran, A. L., Di Maggio, T., Mantella, A., ... Rossolini, G. M. (2015). Methicillin-resistant Staphylococcus aureus in hospitalized patients from the Bolivian Chaco. International Journal of Infectious Diseases, 30, e156-e160. https://doi.org/10.1016/j.ijid.2014.12.006

Methicillin-resistant Staphylococcus aureus in hospitalized patients from the Bolivian Chaco. / Bartoloni, Alessandro; Riccobono, Eleonora; Magnelli, Donata; Villagran, Ana Liz; Di Maggio, Tiziana; Mantella, Antonia; Sennati, Samanta; Revollo, Carmen; Strohmeyer, Marianne; Giani, Tommaso; Pallecchi, Lucia; Rossolini, Gian Maria.

In: International Journal of Infectious Diseases, Vol. 30, 01.01.2015, p. e156-e160.

Research output: Contribution to journalArticle

Bartoloni, A, Riccobono, E, Magnelli, D, Villagran, AL, Di Maggio, T, Mantella, A, Sennati, S, Revollo, C, Strohmeyer, M, Giani, T, Pallecchi, L & Rossolini, GM 2015, 'Methicillin-resistant Staphylococcus aureus in hospitalized patients from the Bolivian Chaco', International Journal of Infectious Diseases, vol. 30, pp. e156-e160. https://doi.org/10.1016/j.ijid.2014.12.006
Bartoloni, Alessandro ; Riccobono, Eleonora ; Magnelli, Donata ; Villagran, Ana Liz ; Di Maggio, Tiziana ; Mantella, Antonia ; Sennati, Samanta ; Revollo, Carmen ; Strohmeyer, Marianne ; Giani, Tommaso ; Pallecchi, Lucia ; Rossolini, Gian Maria. / Methicillin-resistant Staphylococcus aureus in hospitalized patients from the Bolivian Chaco. In: International Journal of Infectious Diseases. 2015 ; Vol. 30. pp. e156-e160.
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abstract = "Objectives: Information is lacking on the methicillin-resistant Staphylococcus aureus (MRSA) clonal lineages circulating in Bolivia. We investigated the prevalence and molecular epidemiology of S. aureus colonization in hospitalized patients from the Bolivian Chaco, and compared their features with those of the few clinical isolates available from that setting. Methods: S. aureus nasal/inguinal colonization was investigated in 280 inpatients from eight hospitals in two point prevalence surveys (2012, n=. 90; 2013, n=. 190). Molecular characterization included genotyping (. spa typing, multilocus sequence typing, and pulsed-field gel electrophoresis), detection of virulence genes, and SCC. mec typing. Results: Forty-one inpatients (14.6{\%}) were S. aureus nasal/inguinal carriers, of whom five were colonized by MRSA (1.8{\%}). MRSA isolates mostly belonged to spa-type t701, harboured SCC. mec IVc, and were negative for Panton-Valentine leukocidin (PVL) genes. However, a USA300-related isolate was also detected, which showed the characteristics of the USA300 Latin American variant (USA300-LV; i.e., ST8, spa-type t008, SCC. mec IVc, presence of PVL genes, absence of arcA). Notably, all the available MRSA clinical isolates (. n=. 5, collected during 2011-2013) were also identified as USA300-LV. Conclusions: Overall, MRSA colonization in inpatients from the Bolivian Chaco was low. However, USA300-LV-related isolates were detected in colonization and infections, emphasizing the importance of implementing control measures to limit their further dissemination in this resource-limited area.",
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