Methisoprinol-effect on the replication cycle of human hepatitis A virus.

M. Divizia; A. Venuti; A. M. Degener; R. Perez-Bercoff; A. Panà

Methisoprinol-effect on the replication cycle of human hepatitis A virus.

M. Divizia, A. Venuti, A. M. Degener, R. Perez-Bercoff, A. Panà

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

The antiviral activity of methisoprinol was investigated under different conditions using a strain of hepatitis A virus (HAV), that shows a strong cytopathic effect on the Frp/3 cell line 7-9 days post-infection. Treatment of Frp/3 at a dose range of 125-1200 micrograms/ml had no toxic effect and showed a dose dependent inhibition of the HAV replication cycle. At the methisoprinol dose of 500 micrograms/ml the cytopathic effect was completely abolished and HAV antigen production reduced by 50% as measured by indirect immunofluorescence (IIF) and commercial enzyme-linked assay (ELISA). The virus yield was virtually abolished at the highest dose employed (1000 micrograms/ml).

Original language	English
Pages (from-to)	323-328
Number of pages	6
Journal	Microbiologica
Volume	15
Issue number	4
Publication status	Published - Oct 1992

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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title = "Methisoprinol-effect on the replication cycle of human hepatitis A virus.",

abstract = "The antiviral activity of methisoprinol was investigated under different conditions using a strain of hepatitis A virus (HAV), that shows a strong cytopathic effect on the Frp/3 cell line 7-9 days post-infection. Treatment of Frp/3 at a dose range of 125-1200 micrograms/ml had no toxic effect and showed a dose dependent inhibition of the HAV replication cycle. At the methisoprinol dose of 500 micrograms/ml the cytopathic effect was completely abolished and HAV antigen production reduced by 50% as measured by indirect immunofluorescence (IIF) and commercial enzyme-linked assay (ELISA). The virus yield was virtually abolished at the highest dose employed (1000 micrograms/ml).",

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AU - Divizia, M.

AU - Venuti, A.

AU - Degener, A. M.

AU - Perez-Bercoff, R.

AU - Panà, A.

PY - 1992/10

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AB - The antiviral activity of methisoprinol was investigated under different conditions using a strain of hepatitis A virus (HAV), that shows a strong cytopathic effect on the Frp/3 cell line 7-9 days post-infection. Treatment of Frp/3 at a dose range of 125-1200 micrograms/ml had no toxic effect and showed a dose dependent inhibition of the HAV replication cycle. At the methisoprinol dose of 500 micrograms/ml the cytopathic effect was completely abolished and HAV antigen production reduced by 50% as measured by indirect immunofluorescence (IIF) and commercial enzyme-linked assay (ELISA). The virus yield was virtually abolished at the highest dose employed (1000 micrograms/ml).

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Methisoprinol-effect on the replication cycle of human hepatitis A virus.

Abstract

ASJC Scopus subject areas

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