Abstract
A "tissue culture" method for testing the cytotoxic activity of compounds active through the formation of metabolites is described and cyclophosphamide (CPA), which is known to exert its effect only after hepatic biotransformation, has been chosen as "model" substance. A monolayer of cultured KB cells is treated for one hour with CPA at 37°C, in the presence of different amounts of microsomal enzymes (105,000 × g fraction) and their co-factors and at the end of the incubation the medium is removed and changed. A dose-effect relation is observed by measuring the inhibition of growth or the destruction of the KB cells after 24 hr. The significance of this procedure for screening purposes or for studying the possible cytotoxicity of unstable metabolites is outlined.
| Original language | English |
|---|---|
| Pages (from-to) | 375-378 |
| Number of pages | 4 |
| Journal | European Journal of Cancer (1965) |
| Volume | 9 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1973 |
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ASJC Scopus subject areas
- Oncology
Cite this
Method for tissue culture evaluation of the cytoxic activity of drugs active through the formation of metabolites. / Dolfini, E.; Martini, A.; Donelli, M. G.; Morasca, L.; Garattini, S.
In: European Journal of Cancer (1965), Vol. 9, No. 5, 1973, p. 375-378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Method for tissue culture evaluation of the cytoxic activity of drugs active through the formation of metabolites
AU - Dolfini, E.
AU - Martini, A.
AU - Donelli, M. G.
AU - Morasca, L.
AU - Garattini, S.
PY - 1973
Y1 - 1973
N2 - A "tissue culture" method for testing the cytotoxic activity of compounds active through the formation of metabolites is described and cyclophosphamide (CPA), which is known to exert its effect only after hepatic biotransformation, has been chosen as "model" substance. A monolayer of cultured KB cells is treated for one hour with CPA at 37°C, in the presence of different amounts of microsomal enzymes (105,000 × g fraction) and their co-factors and at the end of the incubation the medium is removed and changed. A dose-effect relation is observed by measuring the inhibition of growth or the destruction of the KB cells after 24 hr. The significance of this procedure for screening purposes or for studying the possible cytotoxicity of unstable metabolites is outlined.
AB - A "tissue culture" method for testing the cytotoxic activity of compounds active through the formation of metabolites is described and cyclophosphamide (CPA), which is known to exert its effect only after hepatic biotransformation, has been chosen as "model" substance. A monolayer of cultured KB cells is treated for one hour with CPA at 37°C, in the presence of different amounts of microsomal enzymes (105,000 × g fraction) and their co-factors and at the end of the incubation the medium is removed and changed. A dose-effect relation is observed by measuring the inhibition of growth or the destruction of the KB cells after 24 hr. The significance of this procedure for screening purposes or for studying the possible cytotoxicity of unstable metabolites is outlined.
UR - http://www.scopus.com/inward/record.url?scp=0015787295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015787295&partnerID=8YFLogxK
U2 - 10.1016/0014-2964(73)90054-6
DO - 10.1016/0014-2964(73)90054-6
M3 - Article
C2 - 4746736
AN - SCOPUS:0015787295
VL - 9
SP - 375
EP - 378
JO - European Journal of Cancer
JF - European Journal of Cancer
SN - 0014-2964
IS - 5
ER -