Methodological issues in the biological monitoring of urinary benzene and S-phenylmercapturic acid at low exposure levels

Silvia Fustinoni, Laura Campo, Rosa Mercadante, Paola Manini

Research output: Contribution to journalArticlepeer-review

Abstract

Biological monitoring of low level exposure to pollutants is a very challenging analytical activity, and the quality of results is difficult to assess, especially when a certified reference material is unavailable. The aim of this work was to evaluate the reliability of the assays used to measure urinary benzene (Benz-U) and S-phenylmercapturic acid (SPMA), by applying an internal quality control protocol. Urine spot samples from 705 subjects who were either members of the general urban population, gasoline station attendants, or refinery plant workers were assayed for Benz-U and SPMA, using GC/MS and LC/MS/MS, with quantification limits of 15 ng/L and 0.10 μg/L. The median Benz-U concentration was 263 ng/L (60. -2789 ng/L, 5th. -95th percentile), and the median SPMA concentration was 0.19 μg/L (

Original languageEnglish
Pages (from-to)2534-2540
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume878
Issue number27
DOIs
Publication statusPublished - Oct 2010

Keywords

  • Low benzene exposure
  • Quality control
  • Urinary benzene
  • Urinary S-phenylmercapturic acid

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

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