Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology

Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Nicolo Politi, Ilaria Massaiu, Giuliano Mazzini, Giuseppina Micoli, Cinzia Calvio, Maria Gabriella Cusella De Angelis, Paolo Magni

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Whey is an abundant by-product of cheese production process and it is considered a special waste due to its high nutritional load and hypertrophic potential. Technologies for whey valorization are available. They can convert such waste into high-value products, like whey proteins. However, the remaining liquid (called permeate) is still considered as a polluting waste due to its high lactose concentration. The alcoholic fermentation of lactose into ethanol will simultaneously achieve two important goals: safe disposal of a pollutant waste and green energy production. This methodology paper illustrates the workflow carried out to design and realize an optimized microorganism that can efficiently perform the lactose-to-ethanol conversion, engineered via synthetic biology experimental and computational approaches.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages953-956
Number of pages4
Volume2015-November
ISBN (Print)9781424492718
DOIs
Publication statusPublished - Nov 4 2015
Event37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015 - Milan, Italy
Duration: Aug 25 2015Aug 29 2015

Other

Other37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
CountryItaly
CityMilan
Period8/25/158/29/15

Fingerprint

Synthetic Biology
Biocatalysts
Biofuels
Dairies
Lactose
Ethanol
Enzymes
Workflow
Cheese
Cheeses
Fermentation
Waste disposal
Microorganisms
Technology
Byproducts
Proteins
Liquids
Whey

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Pasotti, L., Zucca, S., Casanova, M., Politi, N., Massaiu, I., Mazzini, G., ... Magni, P. (2015). Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (Vol. 2015-November, pp. 953-956). [7318521] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2015.7318521

Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. / Pasotti, Lorenzo; Zucca, Susanna; Casanova, Michela; Politi, Nicolo; Massaiu, Ilaria; Mazzini, Giuliano; Micoli, Giuseppina; Calvio, Cinzia; Cusella De Angelis, Maria Gabriella; Magni, Paolo.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol. 2015-November Institute of Electrical and Electronics Engineers Inc., 2015. p. 953-956 7318521.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pasotti, L, Zucca, S, Casanova, M, Politi, N, Massaiu, I, Mazzini, G, Micoli, G, Calvio, C, Cusella De Angelis, MG & Magni, P 2015, Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. vol. 2015-November, 7318521, Institute of Electrical and Electronics Engineers Inc., pp. 953-956, 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015, Milan, Italy, 8/25/15. https://doi.org/10.1109/EMBC.2015.7318521
Pasotti L, Zucca S, Casanova M, Politi N, Massaiu I, Mazzini G et al. Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol. 2015-November. Institute of Electrical and Electronics Engineers Inc. 2015. p. 953-956. 7318521 https://doi.org/10.1109/EMBC.2015.7318521
Pasotti, Lorenzo ; Zucca, Susanna ; Casanova, Michela ; Politi, Nicolo ; Massaiu, Ilaria ; Mazzini, Giuliano ; Micoli, Giuseppina ; Calvio, Cinzia ; Cusella De Angelis, Maria Gabriella ; Magni, Paolo. / Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol. 2015-November Institute of Electrical and Electronics Engineers Inc., 2015. pp. 953-956
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