Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and Its Clinical Applications

Antonella Zucchetto, Erika Tissino, Alexander Chigaev, Tanja Nicole Hartmann, Valter Gattei

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The integrin heterodimer CD49d/CD29 (a.k.a. Very Late Antigen-4, VLA-4) mediates cell-cell and cell-matrix interaction through the binding of its ligands VCAM-1 and fibronectin. VLA-4 can be present on the cell surface at different conformation states that affect the binding affinity for the ligands. In chronic lymphocytic leukemia (CLL), higher VLA-4 levels, as determined by measuring the expression of CD49d chain by flow cytometry, have been demonstrated to associate with a worse prognosis, in keeping with the role of VLA-4 as key molecule favoring CLL cell localization in protective niches of bone marrow and lymph nodes. Given the emerging clinical relevance of VLA-4 evaluation in CLL, both in the setting of the conventional chemo-immunotherapy and the novel drugs targeting the BCR pathway, here we describe the flow cytometric approaches followed by us to quantify the CD49d expression levels and the VLA-4 activation status in CLL cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages101-112
Number of pages12
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1881
ISSN (Print)1064-3745

Keywords

  • CD49d expression
  • Flow cytometry
  • HUTS-21 antibody
  • Integrin
  • VLA-4 activation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Zucchetto, A., Tissino, E., Chigaev, A., Hartmann, T. N., & Gattei, V. (2019). Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and Its Clinical Applications. In Methods in Molecular Biology (pp. 101-112). (Methods in Molecular Biology; Vol. 1881). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8876-1_8