This review summarizes the clinical significance and the mode of execution of many non-invasive or minimally invasive tests to characterize the activity of cytochrome P450 microsomal enzymes. The article takes into consideration non-specific indicators of oxidative capacity (antipyrine, aminopyrine) as well as relatively selective markers of cytochromes CYP1A2 (phenacetin, theophylline, caffeine), CYP2C9 (phenytoin), CYP2C18 (mephenytoin), CYP2D6 (dextrometorphan, debrisoquine, sparteine), CYP2E1 (chlorzoxazone) and CYP3A (6-beta-hydroxycortisol, erythromycin, dapsone, ethosuximide). The determination of these markers may be useful to evaluate individual capacity to metabolize several drugs which are substrates of cytochrome P450 isoenzymes. The same tests may also be used to investigate factors affecting the activity of these isoenzymes, in order to predict important drug interactions or metabolic alterations which modify individual susceptibility to the toxic effects of various drugs and environmental contaminants.
|Translated title of the contribution||Methods for the evaluation of oxidative enzyme activity involved in drug metabolism: Clinical significance and technical aspects|
|Number of pages||13|
|Journal||Giornale Italiano di Chimica Clinica|
|Publication status||Published - 1994|
ASJC Scopus subject areas
- Clinical Biochemistry