Methods for urinary testosterone analysis

Elisabetta Venturelli, Adalberto Cavalleri, Giorgio Secreto

Research output: Contribution to journalArticlepeer-review


Urinary testosterone analysis requires a multistep procedure to achieve a good degree of sensitivity and specificity in the dosage. Hydrolysis, extraction, purification and quantification are usually performed in sequence, and several options can be chosen for each of them. After introductory remarks on the applications of urinary testosterone measurement and a short description of the metabolic pathway of the hormone, an overview of the techniques most commonly used in each step is presented. Advantages and disadvantages of each of them are outlined, and a procedure for urinary testosterone analysis is suggested. The procedure consists of: enzymatic hydrolysis with Helix pomatia juice, followed by solid-phase extraction of hydrolyzed urine by a C18 cartridge coupled with an NH2 cartridge and high-performance liquid chromatography cleanup of the extract. Then, quantification can be achieved by gas chromatography or radioimmunoassay.

Original languageEnglish
Pages (from-to)363-380
Number of pages18
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number1-2
Publication statusPublished - Sep 15 1995

ASJC Scopus subject areas

  • Chemistry(all)


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