Methylated RASSF1A in malignant peripheral nerve sheath tumors identifies neurofibromatosis type 1 patients with inferior prognosis

Stine A. Danielsen, Guro E. Lind, Matthias Kolberg, Maren Høland, Bodil Bjerkehagen, Kirsten Sundby Hall, Eva Van Den Berg, Fredrik Mertens, Sigbjørn Smeland, Piero Picci, Ragnhild A. Lothe

Research output: Contribution to journalArticlepeer-review

Abstract

Background Malignant peripheral nerve sheath tumor (MPNST) is a rare and highly aggressive disease with no evidence of effect from adjuvant therapy. It is further associated with the hereditary syndrome neurofibromatosis type 1 (NF1). Silencing of the tumor suppressor gene RASSF1A through DNA promoter hypermethylation is known to be involved in cancer development, but its impact in MPNSTs remains unsettled. Methods The RASSF1A promoter was analyzed by methylation-specific PCR in 113 specimens, including 44 NF1-associated MPNSTs, 47 sporadic MPNSTs, 21 benign neurofibromas, and 1 nonneoplastic nerve sheath control. Results RASSF1A methylation was found only in the malignant samples (60%) and identified a subgroup among patients with NF1-associated MPNST with a poor prognosis. These patients had a mean 5-year disease-specific survival of 27.3 months (95% CI: 17.2-37.4) versus 47.4 months (95% CI: 37.5-57.2) for NF1 patients with unmethylated promoters, P = 0.014. In multivariate Cox regression analysis, methylated RASSF1A remained an adverse prognostic factor independent of clinical risk factors, P =. 013 (hazard ratio: 5.2; 95% CI: 1.4-19.4). Conclusion A considerable number of MPNST samples display hypermethylation of the RASSF1A gene promoter, and for these tumors, this is the first molecular marker that if validated can characterize a subgroup of patients with inferior prognosis, restricted to individuals with NF1.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalNeuro-Oncology
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Methylation
  • MPNST
  • NF1
  • RASSF1A
  • Survival

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

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