Methylation analysis of HOXA10 regulatory elements in patients with endometriosis

Pietro G. Signorile, Anna Severino, Massimo Santoro, Maria Spyrou, Rosa Viceconte, Alfonso Baldi

Research output: Contribution to journalArticle

Abstract

Objective: The pathogenesis of endometriosis is still mysterious, being retrograde menstruation and coelomic metaplasia the most accepted hypotheses. Recently, it has been proposed that endometriosis is caused by fine-tuning alterations of the female genital system development during the foetal life and that in utero exposition to endocrine disruptors can be one of the factors causing the disease, possibly acting on the methylation status of the genome. In this study, we have evaluated the methylation status of HOXA10 gene regulation regions in a cohort of 22 endometriosis patients respect to a control group of 6 healthy women. Results: The methylation study was carried out on three CpG islands, previously described hypermethylated in the endometrium of endometriosis patients and include 22 CpG sites, 21 CpG sites and 10 CpG sites, respectively identified through the online platform MethPrimer. The analysis did not find significant differences between patients with endometriosis and healthy control individuals. These results confirm previous studies on genome wide methylation analysis in endometriosis patients. Therefore, other epigenetically altered genes should be considered more related to the pathogenesis of endometriosis.

Original languageEnglish
Article number722
JournalBMC Research Notes
Volume11
Issue number1
DOIs
Publication statusPublished - Oct 11 2018

Keywords

  • CpG islands
  • DNA methylation analysis
  • Endometriosis
  • HOXA10 gene promoter

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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