Methylation analysis of multiple genes in blood DNA of Alzheimer's disease and healthy individuals

Pierpaola Tannorella, Andrea Stoccoro, Gloria Tognoni, Lucia Petrozzi, Maria Grazia Salluzzo, Alda Ragalmuto, Gabriele Siciliano, Alexander Haslberger, Paolo Bosco, Ubaldo Bonuccelli, Lucia Migliore, Fabio Coppedè

Research output: Contribution to journalArticlepeer-review

Abstract

We collected blood DNA from 120 late-onset Alzheimer's disease (AD) patients and 115 healthy matched controls and analysed the methylation levels of genes involved in amyloid-beta peptide production (PSEN1 and BACE1), in DNA methylation (DNMT1, DNMT3A and DNMT3B), and in one-carbon metabolism (MTHFR), searching for correlation with age and gender, with biomarkers of one-carbon metabolism (plasma homocysteine, and serum folate and vitamin B12 levels), and with disease status (being healthy or having AD). We also evaluated the contribution of the APOE ε4 allele, the major late-onset AD genetic risk factor, to the studied gene methylation levels. All the genes showed low mean methylation levels (

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalNeuroscience Letters
Volume600
DOIs
Publication statusPublished - 2015

Keywords

  • Alzheimer's disease
  • APOE
  • BACE1
  • DNA methylation
  • DNMTs
  • Folate
  • Homocysteine
  • MTHFR
  • PSEN1
  • Vitamin B12

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Fingerprint

Dive into the research topics of 'Methylation analysis of multiple genes in blood DNA of Alzheimer's disease and healthy individuals'. Together they form a unique fingerprint.

Cite this