Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells

Valentina Bianchessi, Maria Cristina Vinci, Patrizia Nigro, Valeria Rizzi, Floriana Farina, Maurizio Capogrossi, Giulio Pompilio, Valentina Gualdi, Andrea Lauri

Research output: Contribution to journalArticlepeer-review

Abstract

The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.

Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalMitochondrion
Volume27
DOIs
Publication statusPublished - Mar 1 2016

Keywords

  • Bisulfite sequencing
  • D-loop
  • Endothelial Cell
  • Methylation
  • MtDNA
  • Senescence

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine

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