TY - JOUR
T1 - Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells
AU - Bianchessi, Valentina
AU - Vinci, Maria Cristina
AU - Nigro, Patrizia
AU - Rizzi, Valeria
AU - Farina, Floriana
AU - Capogrossi, Maurizio
AU - Pompilio, Giulio
AU - Gualdi, Valentina
AU - Lauri, Andrea
PY - 2016/3/1
Y1 - 2016/3/1
N2 - The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.
AB - The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.
KW - Bisulfite sequencing
KW - D-loop
KW - Endothelial Cell
KW - Methylation
KW - MtDNA
KW - Senescence
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U2 - 10.1016/j.mito.2016.02.004
DO - 10.1016/j.mito.2016.02.004
M3 - Article
VL - 27
SP - 40
EP - 47
JO - Mitochondrion
JF - Mitochondrion
SN - 1567-7249
ER -