Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells

Valentina Bianchessi; Maria Cristina Vinci; Patrizia Nigro; Valeria Rizzi; Floriana Farina; Maurizio Capogrossi; Giulio Pompilio; Valentina Gualdi; Andrea Lauri

doi:10.1016/j.mito.2016.02.004

Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells

Valentina Bianchessi, Maria Cristina Vinci, Patrizia Nigro, Valeria Rizzi, Floriana Farina, Maurizio Capogrossi, Giulio Pompilio, Valentina Gualdi, Andrea Lauri

Istituto Dermopatico dell'Immacolata , IDI

Research output: Contribution to journal › Article › peer-review

Abstract

The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.

Original language	English
Pages (from-to)	40-47
Number of pages	8
Journal	Mitochondrion
Volume	27
DOIs	https://doi.org/10.1016/j.mito.2016.02.004
Publication status	Published - Mar 1 2016

Keywords

Bisulfite sequencing
D-loop
Endothelial Cell
Methylation
MtDNA
Senescence

ASJC Scopus subject areas

Cell Biology
Molecular Biology
Molecular Medicine

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10.1016/j.mito.2016.02.004

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title = "Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells",

abstract = "The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.",

keywords = "Bisulfite sequencing, D-loop, Endothelial Cell, Methylation, MtDNA, Senescence",

author = "Valentina Bianchessi and Vinci, {Maria Cristina} and Patrizia Nigro and Valeria Rizzi and Floriana Farina and Maurizio Capogrossi and Giulio Pompilio and Valentina Gualdi and Andrea Lauri",

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doi = "10.1016/j.mito.2016.02.004",

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T1 - Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells

AU - Bianchessi, Valentina

AU - Vinci, Maria Cristina

AU - Nigro, Patrizia

AU - Rizzi, Valeria

AU - Farina, Floriana

AU - Capogrossi, Maurizio

AU - Pompilio, Giulio

AU - Gualdi, Valentina

AU - Lauri, Andrea

PY - 2016/3/1

Y1 - 2016/3/1

N2 - The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.

AB - The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression.

KW - Bisulfite sequencing

KW - D-loop

KW - Endothelial Cell

KW - Methylation

KW - MtDNA

KW - Senescence

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Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells

Abstract

Keywords

ASJC Scopus subject areas

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