TY - JOUR
T1 - MG-63 human osteosarcoma cells grown in monolayer and as three-dimensional tumor spheroids present a different metabolic profile
T2 - A 1H NMR study
AU - Santini, Maria Teresa
AU - Rainaldi, Gabriella
AU - Romano, Rocco
AU - Ferrante, Antonella
AU - Clemente, Stefania
AU - Motta, Andrea
AU - Indovina, Pietro Luigi
PY - 2004/1/16
Y1 - 2004/1/16
N2 - High resolution proton nuclear magnetic resonance (1H NMR) spectroscopy was used to determine if the same cell line (MG-63 human osteosarcoma cells) grown in monolayer or as small (about 50-80 μm in diameter), three-dimensional tumor spheroids with no hypoxic center has different metabolic characteristics. Consequently, the 1H NMR spectra were obtained from both types of cultures and then compared. The results indicate that the type of cellular spatial array determines specific changes in MG-63 cells. In particular, small but significant differences in lactate and alanine indicating a perturbation in energy metabolism were observed in the two cell models. In addition, although variations in CH 2 and CH3 groups were also seen, it is not possible at this time to establish if lipid metabolism is truly different in cells and spheroids.
AB - High resolution proton nuclear magnetic resonance (1H NMR) spectroscopy was used to determine if the same cell line (MG-63 human osteosarcoma cells) grown in monolayer or as small (about 50-80 μm in diameter), three-dimensional tumor spheroids with no hypoxic center has different metabolic characteristics. Consequently, the 1H NMR spectra were obtained from both types of cultures and then compared. The results indicate that the type of cellular spatial array determines specific changes in MG-63 cells. In particular, small but significant differences in lactate and alanine indicating a perturbation in energy metabolism were observed in the two cell models. In addition, although variations in CH 2 and CH3 groups were also seen, it is not possible at this time to establish if lipid metabolism is truly different in cells and spheroids.
KW - High resolution nuclear magnetic resonance spectroscopy
KW - Human osteosarcoma cell
KW - Monolayer culture
KW - Three-dimensional tumor spheroid
UR - http://www.scopus.com/inward/record.url?scp=1642450627&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642450627&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(03)01466-2
DO - 10.1016/S0014-5793(03)01466-2
M3 - Article
C2 - 14741358
AN - SCOPUS:1642450627
VL - 557
SP - 148
EP - 154
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -