mGlu1α receptors are co-expressed with CB1 receptors in a subset of interneurons in the CA1 region of organotypic hippocampal slice cultures and adult rat brain

Francesca Boscia, Francesco Ferraguti, Flavio Moroni, Lucio Annunziato, Domenico E. Pellegrini-Giampietro

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recent studies have demonstrated a functional interaction between group I metabotropic glutamate (mGlu) receptors and the cannabinoid system in the modulation of synaptic transmission. By using antisera directed against mGlu1α and CB1 cannabinoid receptors, we examined their distribution in the CA1 region of rat organotypic hippocampal slice cultures. Immunoreactive mGlu1α and CB1 elements were localized in non-principal cells, with a labeling distribution that was very similar to the pattern previously observed in the adult rat brain. Double-immunofluorescence staining and confocal microscopy showed that a subset of interneurons, mainly located in the stratum radiatum, was double-labeled for both mGlu1α and CB1 receptors. Co-localization of the two receptor subtypes was confirmed in hippocampal sections from adult rat brain. By using the "mirror technique" in adjacent sections, we observed that the double-labeled cells for mGlu1α and CB1 receptors were also immunopositive for the cholecystokinin peptide. Quantitative analysis revealed that in the stratum radiatum the majority (92%) of the CB1-positive cells and 19% of the mGlu1α-positive cells expressed both receptors. Triple immunofluorescence staining showed partial co-labeling of mGlu1α- and CB1-immunopositive cells with the vesicular glutamate transporter 3 or calbindin. Our results demonstrate that mGlu1α and CB1 receptors co-exist in a subpopulation of inhibitory neurons in the stratum radiatum of the hippocampus that is suggestive of the Schaffer collateral-associated interneurons. Hence, additional functional mechanisms underlying the cooperation between these two receptor subtypes may exist.

Original languageEnglish
Pages (from-to)428-439
Number of pages12
JournalNeuropharmacology
Volume55
Issue number4
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Hippocampal CA1 Region
Cannabinoid Receptor CB1
Interneurons
Brain
Hippocampus
S100 Calcium Binding Protein G
Vesicular Glutamate Transport Proteins
Staining and Labeling
Metabotropic Glutamate Receptors
Cannabinoids
Cholecystokinin
Fluorescence Microscopy
Confocal Microscopy
Synaptic Transmission
Fluorescent Antibody Technique
Immune Sera
Neurons
Peptides

Keywords

  • Calbindin
  • Cannabinoid receptors
  • Cholecystokinin
  • Endocannabinoids
  • Metabotropic glutamate receptors
  • VGluT3

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

mGlu1α receptors are co-expressed with CB1 receptors in a subset of interneurons in the CA1 region of organotypic hippocampal slice cultures and adult rat brain. / Boscia, Francesca; Ferraguti, Francesco; Moroni, Flavio; Annunziato, Lucio; Pellegrini-Giampietro, Domenico E.

In: Neuropharmacology, Vol. 55, No. 4, 09.2008, p. 428-439.

Research output: Contribution to journalArticle

Boscia, Francesca ; Ferraguti, Francesco ; Moroni, Flavio ; Annunziato, Lucio ; Pellegrini-Giampietro, Domenico E. / mGlu1α receptors are co-expressed with CB1 receptors in a subset of interneurons in the CA1 region of organotypic hippocampal slice cultures and adult rat brain. In: Neuropharmacology. 2008 ; Vol. 55, No. 4. pp. 428-439.
@article{7010fc0e29cf4ca89bf06ab34fd10f96,
title = "mGlu1α receptors are co-expressed with CB1 receptors in a subset of interneurons in the CA1 region of organotypic hippocampal slice cultures and adult rat brain",
abstract = "Recent studies have demonstrated a functional interaction between group I metabotropic glutamate (mGlu) receptors and the cannabinoid system in the modulation of synaptic transmission. By using antisera directed against mGlu1α and CB1 cannabinoid receptors, we examined their distribution in the CA1 region of rat organotypic hippocampal slice cultures. Immunoreactive mGlu1α and CB1 elements were localized in non-principal cells, with a labeling distribution that was very similar to the pattern previously observed in the adult rat brain. Double-immunofluorescence staining and confocal microscopy showed that a subset of interneurons, mainly located in the stratum radiatum, was double-labeled for both mGlu1α and CB1 receptors. Co-localization of the two receptor subtypes was confirmed in hippocampal sections from adult rat brain. By using the {"}mirror technique{"} in adjacent sections, we observed that the double-labeled cells for mGlu1α and CB1 receptors were also immunopositive for the cholecystokinin peptide. Quantitative analysis revealed that in the stratum radiatum the majority (92{\%}) of the CB1-positive cells and 19{\%} of the mGlu1α-positive cells expressed both receptors. Triple immunofluorescence staining showed partial co-labeling of mGlu1α- and CB1-immunopositive cells with the vesicular glutamate transporter 3 or calbindin. Our results demonstrate that mGlu1α and CB1 receptors co-exist in a subpopulation of inhibitory neurons in the stratum radiatum of the hippocampus that is suggestive of the Schaffer collateral-associated interneurons. Hence, additional functional mechanisms underlying the cooperation between these two receptor subtypes may exist.",
keywords = "Calbindin, Cannabinoid receptors, Cholecystokinin, Endocannabinoids, Metabotropic glutamate receptors, VGluT3",
author = "Francesca Boscia and Francesco Ferraguti and Flavio Moroni and Lucio Annunziato and Pellegrini-Giampietro, {Domenico E.}",
year = "2008",
month = "9",
doi = "10.1016/j.neuropharm.2008.04.024",
language = "English",
volume = "55",
pages = "428--439",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - mGlu1α receptors are co-expressed with CB1 receptors in a subset of interneurons in the CA1 region of organotypic hippocampal slice cultures and adult rat brain

AU - Boscia, Francesca

AU - Ferraguti, Francesco

AU - Moroni, Flavio

AU - Annunziato, Lucio

AU - Pellegrini-Giampietro, Domenico E.

PY - 2008/9

Y1 - 2008/9

N2 - Recent studies have demonstrated a functional interaction between group I metabotropic glutamate (mGlu) receptors and the cannabinoid system in the modulation of synaptic transmission. By using antisera directed against mGlu1α and CB1 cannabinoid receptors, we examined their distribution in the CA1 region of rat organotypic hippocampal slice cultures. Immunoreactive mGlu1α and CB1 elements were localized in non-principal cells, with a labeling distribution that was very similar to the pattern previously observed in the adult rat brain. Double-immunofluorescence staining and confocal microscopy showed that a subset of interneurons, mainly located in the stratum radiatum, was double-labeled for both mGlu1α and CB1 receptors. Co-localization of the two receptor subtypes was confirmed in hippocampal sections from adult rat brain. By using the "mirror technique" in adjacent sections, we observed that the double-labeled cells for mGlu1α and CB1 receptors were also immunopositive for the cholecystokinin peptide. Quantitative analysis revealed that in the stratum radiatum the majority (92%) of the CB1-positive cells and 19% of the mGlu1α-positive cells expressed both receptors. Triple immunofluorescence staining showed partial co-labeling of mGlu1α- and CB1-immunopositive cells with the vesicular glutamate transporter 3 or calbindin. Our results demonstrate that mGlu1α and CB1 receptors co-exist in a subpopulation of inhibitory neurons in the stratum radiatum of the hippocampus that is suggestive of the Schaffer collateral-associated interneurons. Hence, additional functional mechanisms underlying the cooperation between these two receptor subtypes may exist.

AB - Recent studies have demonstrated a functional interaction between group I metabotropic glutamate (mGlu) receptors and the cannabinoid system in the modulation of synaptic transmission. By using antisera directed against mGlu1α and CB1 cannabinoid receptors, we examined their distribution in the CA1 region of rat organotypic hippocampal slice cultures. Immunoreactive mGlu1α and CB1 elements were localized in non-principal cells, with a labeling distribution that was very similar to the pattern previously observed in the adult rat brain. Double-immunofluorescence staining and confocal microscopy showed that a subset of interneurons, mainly located in the stratum radiatum, was double-labeled for both mGlu1α and CB1 receptors. Co-localization of the two receptor subtypes was confirmed in hippocampal sections from adult rat brain. By using the "mirror technique" in adjacent sections, we observed that the double-labeled cells for mGlu1α and CB1 receptors were also immunopositive for the cholecystokinin peptide. Quantitative analysis revealed that in the stratum radiatum the majority (92%) of the CB1-positive cells and 19% of the mGlu1α-positive cells expressed both receptors. Triple immunofluorescence staining showed partial co-labeling of mGlu1α- and CB1-immunopositive cells with the vesicular glutamate transporter 3 or calbindin. Our results demonstrate that mGlu1α and CB1 receptors co-exist in a subpopulation of inhibitory neurons in the stratum radiatum of the hippocampus that is suggestive of the Schaffer collateral-associated interneurons. Hence, additional functional mechanisms underlying the cooperation between these two receptor subtypes may exist.

KW - Calbindin

KW - Cannabinoid receptors

KW - Cholecystokinin

KW - Endocannabinoids

KW - Metabotropic glutamate receptors

KW - VGluT3

UR - http://www.scopus.com/inward/record.url?scp=49949098675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49949098675&partnerID=8YFLogxK

U2 - 10.1016/j.neuropharm.2008.04.024

DO - 10.1016/j.neuropharm.2008.04.024

M3 - Article

C2 - 18599092

AN - SCOPUS:49949098675

VL - 55

SP - 428

EP - 439

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 4

ER -