Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability

Teresa Odorisio, Cataldo Schietroma, M. Letizia Zaccaria, Francesca Cianfarani, Cecilia Tiveron, Laura Talangelo, Cristina M. Failla, Giovanna Zambruno

Research output: Contribution to journalArticle

Abstract

Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.

Original languageEnglish
Pages (from-to)2559-2567
Number of pages9
JournalJournal of Cell Science
Volume115
Issue number12
Publication statusPublished - Jun 15 2002

Fingerprint

Permeability
Skin
Vascular Endothelial Growth Factor A
Transgenic Mice
Lymphatic Endothelium
Keratin-14
Cytokines
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
Vascular Endothelium
Capillary Permeability
Chemotaxis
Endothelium
Blood Vessels
Monocytes
Placenta Growth Factor

Keywords

  • Angiogenesis
  • PIGF
  • Transgenic mice
  • VEGF
  • Vessel permeability

ASJC Scopus subject areas

  • Cell Biology

Cite this

Odorisio, T., Schietroma, C., Zaccaria, M. L., Cianfarani, F., Tiveron, C., Talangelo, L., ... Zambruno, G. (2002). Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability. Journal of Cell Science, 115(12), 2559-2567.

Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability. / Odorisio, Teresa; Schietroma, Cataldo; Zaccaria, M. Letizia; Cianfarani, Francesca; Tiveron, Cecilia; Talangelo, Laura; Failla, Cristina M.; Zambruno, Giovanna.

In: Journal of Cell Science, Vol. 115, No. 12, 15.06.2002, p. 2559-2567.

Research output: Contribution to journalArticle

Odorisio, T, Schietroma, C, Zaccaria, ML, Cianfarani, F, Tiveron, C, Talangelo, L, Failla, CM & Zambruno, G 2002, 'Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability', Journal of Cell Science, vol. 115, no. 12, pp. 2559-2567.
Odorisio T, Schietroma C, Zaccaria ML, Cianfarani F, Tiveron C, Talangelo L et al. Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability. Journal of Cell Science. 2002 Jun 15;115(12):2559-2567.
Odorisio, Teresa ; Schietroma, Cataldo ; Zaccaria, M. Letizia ; Cianfarani, Francesca ; Tiveron, Cecilia ; Talangelo, Laura ; Failla, Cristina M. ; Zambruno, Giovanna. / Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability. In: Journal of Cell Science. 2002 ; Vol. 115, No. 12. pp. 2559-2567.
@article{f30b317884d543e3874b83be467df6fc,
title = "Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability",
abstract = "Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.",
keywords = "Angiogenesis, PIGF, Transgenic mice, VEGF, Vessel permeability",
author = "Teresa Odorisio and Cataldo Schietroma and Zaccaria, {M. Letizia} and Francesca Cianfarani and Cecilia Tiveron and Laura Talangelo and Failla, {Cristina M.} and Giovanna Zambruno",
year = "2002",
month = "6",
day = "15",
language = "English",
volume = "115",
pages = "2559--2567",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "12",

}

TY - JOUR

T1 - Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability

AU - Odorisio, Teresa

AU - Schietroma, Cataldo

AU - Zaccaria, M. Letizia

AU - Cianfarani, Francesca

AU - Tiveron, Cecilia

AU - Talangelo, Laura

AU - Failla, Cristina M.

AU - Zambruno, Giovanna

PY - 2002/6/15

Y1 - 2002/6/15

N2 - Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.

AB - Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.

KW - Angiogenesis

KW - PIGF

KW - Transgenic mice

KW - VEGF

KW - Vessel permeability

UR - http://www.scopus.com/inward/record.url?scp=0037096168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037096168&partnerID=8YFLogxK

M3 - Article

C2 - 12045226

AN - SCOPUS:0037096168

VL - 115

SP - 2559

EP - 2567

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 12

ER -