Abstract
Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.
| Original language | English |
|---|---|
| Pages (from-to) | 2559-2567 |
| Number of pages | 9 |
| Journal | Journal of Cell Science |
| Volume | 115 |
| Issue number | 12 |
| Publication status | Published - Jun 15 2002 |
Fingerprint
Keywords
- Angiogenesis
- PIGF
- Transgenic mice
- VEGF
- Vessel permeability
ASJC Scopus subject areas
- Cell Biology
Cite this
Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability. / Odorisio, Teresa; Schietroma, Cataldo; Zaccaria, M. Letizia; Cianfarani, Francesca; Tiveron, Cecilia; Talangelo, Laura; Failla, Cristina M.; Zambruno, Giovanna.
In: Journal of Cell Science, Vol. 115, No. 12, 15.06.2002, p. 2559-2567.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability
AU - Odorisio, Teresa
AU - Schietroma, Cataldo
AU - Zaccaria, M. Letizia
AU - Cianfarani, Francesca
AU - Tiveron, Cecilia
AU - Talangelo, Laura
AU - Failla, Cristina M.
AU - Zambruno, Giovanna
PY - 2002/6/15
Y1 - 2002/6/15
N2 - Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.
AB - Placenta growth factor (PIGF) is a member of the vascular endothelial growth factor (VEGF) family, comprising at least five cytokines specifically involved in the regulation of vascular and/or lymphatic endothelium differentiation. Several lines of evidence indicate a role for PIGF in monocyte chemotaxis and in potentiating the activity of VEGF, but the exact function of this cytokine is not fully understood. To define the biological role of PIGF in vivo, we have produced a transgenic mouse model overexpressing this factor in the skin by using a keratin 14 promoter cassette. Our data indicate that PIGF has strong angiogenic properties in both fetal and adult life. PIGF overexpression results in a substantial increase in the number, branching and size of dermal blood vessels as well as in enhanced vascular permeability. Indeed, intradermally injected recombinant PIGF was able to induce vessel permeability in wild-type mice. The analysis of vascular endothelial growth factor receptor 1/flt-1 and vascular endothelial growth factor receptor 2/flk-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PIGF.
KW - Angiogenesis
KW - PIGF
KW - Transgenic mice
KW - VEGF
KW - Vessel permeability
UR - http://www.scopus.com/inward/record.url?scp=0037096168&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037096168&partnerID=8YFLogxK
M3 - Article
C2 - 12045226
AN - SCOPUS:0037096168
VL - 115
SP - 2559
EP - 2567
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 12
ER -