Micellar electrokinetic capillary chromatography of three natural vitamin A derivatives

Aldo Profumo, Vincenzo Profumo, Giorgio Vidali

Research output: Contribution to journalArticle

Abstract

Micellar electrokinetic chromatography (MEKC) using bile salts has been employed to separate retinoids differing in structure and charge; bile salts in MEKC allows the separation of liposoluble molecules but, to the best of our knowledge, there are only few data on the above-mentioned technique for the separation of highly hydrophobic compounds. The three natural vitamin A derivatives, retinal, retinol and retinoic acid, were successfully separated by MEKC using sodium cholate within a relatively short time (ca. 25 min), whereas the separation of these compounds was not successful using sodium dodecyl sulfate or sodium deoxycholate. Several parameters (pH and organic modifiers, in addition to bile salts concentration) have been tested to provide a system that can be extended to synthetic retinoids, which are often used in treating several diseases, including cancer prevention and therapy.

Original languageEnglish
Pages (from-to)1617-1621
Number of pages5
JournalElectrophoresis
Volume17
Issue number10
DOIs
Publication statusPublished - Oct 1996

Keywords

  • Bile salts
  • Capillary electrophoresis
  • Micelles
  • Retinoids

ASJC Scopus subject areas

  • Clinical Biochemistry

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