Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state-of-the-art

Paolo Iadarola, Giuseppe Cetta, Maurizio Luisetti, Laura Annovazzi, Begona Casado, James Baraniuk, Chiara Zanone, Simona Viglio

Research output: Contribution to journalArticlepeer-review

Abstract

Human urine plays a central role in clinical diagnostic being one of the most-frequently used body fluid for detection of biological markers. Samples from patients with different diseases display patterns of biomarkers that differ significantly from those obtained from healthy subjects. The availability of fast, reproducible, and easy-to-apply analytical techniques that would allow identification of a large number of these analytes is thus highly desiderable since they may provide detailed information about the progression of a pathological process. From among the variety of methods so far applied for the determination of urinary metabolites, capillary electrophoresis, both in the capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) modes, represents a robust and reliable analytical tool widely used in this area. The aim of the present article is to focus the interest of the reader on recent applications of MEKC and CZE in the field of urinary biomarkers and to discuss advantages and/or limitations of each mode.

Original languageEnglish
Pages (from-to)752-766
Number of pages15
JournalElectrophoresis
Volume26
Issue number4-5
DOIs
Publication statusPublished - Feb 2005

Keywords

  • Biomarkers
  • Capillary zone electrophoresis
  • Human urine
  • Micellar electrokinetic chromatography
  • Review

ASJC Scopus subject areas

  • Clinical Biochemistry

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