Micellar lipid composition profoundly affects LXR-dependent cholesterol transport across CaCo2 cells

Michele Petruzzelli, Albert K. Groen, Karel J. van Erpecum, Carlos Vrins, Astrid E. van der Velde, Piero Portincasa, Giuseppe Palasciano, Gerard P. van Berge Henegouwen, Giuseppe Lo Sasso, Annalisa Morgano, Antonio Moschetta

Research output: Contribution to journalArticlepeer-review

Abstract

Intraluminal phospholipids affect micellar solubilization and absorption of cholesterol. We here study cholesterol transport from taurocholate-phospholipid-cholesterol micelles to CaCo2 cells, and associated effects on ABC-A1 mediated cholesterol efflux. Micellar incorporation of egg-yolk-phosphatidylcholine markedly increased apical retention of the sterol with decreased expression of ABC-A1, an effect that is prevented by synthetic liver X receptor (LXR) or retinoid X receptor (RXR) agonists. On the other hand, incorporation of lyso-phosphatidylcholine (LysoPC) increased ABC-A1-HDL-dependent basolateral cholesterol efflux, an effect that is abated when LXR is silenced. Thus, the modulation of cholesterol metabolism via intraluminal phospholipids is related to the activity of the oxysterol nuclear receptor LXR.

Original languageEnglish
Pages (from-to)1274-1280
Number of pages7
JournalFEBS Letters
Volume583
Issue number8
DOIs
Publication statusPublished - Apr 17 2009

Keywords

  • ABC-A1
  • Bile salt
  • Intestine
  • Liver X receptor
  • Lyso-phosphatidylcholine
  • Phosphatidylcholine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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