Micro-CT technique for three-dimensional visualization of human stem cells

Andrea Farini, Chiara Villa, Marzia Belicchi, Mirella Meregalli, Yvan Torrente

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Micro-CT offers high spatial resolution of the distribution of stem cells and provides rapid reconstruction of 3D images and quantitative volumetric analysis. Together with real-time PCR analysis, micro-CT offers the possibility to obtain a quantification of the number of cells that are able to migrate from the bloodstream inside the muscular tissues. Here, we studied for the first time the kinetics of the human cells injected into the femoral artery of DMD animal model. It is fundamental to determine whether the cells disseminate and entrap only within the capillary system of downstream muscles and/or they are able to reach the non-injected muscles and other organs through blood flow. The efficient transplantation of stem cells to dystrophic-deficient muscle reinforced the utility of intra-arterial delivery of cells as a viable approach for cell-based clinical therapies of neuromuscular diseases.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages143-152
Number of pages10
Volume1052
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1052
ISSN (Print)10643745

Keywords

  • 3D images
  • CD133+ stem cells
  • Micro-CT
  • Muscle homing
  • Muscular dystrophy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Farini, A., Villa, C., Belicchi, M., Meregalli, M., & Torrente, Y. (2013). Micro-CT technique for three-dimensional visualization of human stem cells. In Methods in Molecular Biology (Vol. 1052, pp. 143-152). (Methods in Molecular Biology; Vol. 1052). https://doi.org/10.1007/7651-2013-23