Micro-injection of recombinant lysyl oxidase blocks oncogenic p21-Ha- Ras and progesterone effects on Xenopus laevis oocyte maturation

Armando Di Donato, Juan Carlos Lacal, Marco Di Duca, Monia Giampuzzi, Gianmarco Ghiggeri, Rosanna Gusmano

Research output: Contribution to journalArticlepeer-review

Abstract

Previous evidence suggested an anti-oncogenic role for lysyl oxidase, mainly in ras-transformed cells. Here we prove that recombinant lysyl oxidase is actually able to antagonize p21-Ha-Ras-induced Xenopus laevis oocyte maturation. Lysyl oxidase was also effective on progesterone-dependent maturation, indicating a block lying downstream of Ras. Maturation induced by activated 'maturation promoting factor', normally triggered by progesterone, was also inhibited by lysyl oxidase. Finally, lysyl oxidase did not abolish p42(Erk2) phosphorylation upon maturation triggering, suggesting a block downstream of Erk2. Further investigation showed that lysyl oxidase action depends on protein synthesis and is therefore probably mediated by a newly synthesized protein.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalFEBS Letters
Volume419
Issue number1
DOIs
Publication statusPublished - Dec 8 1997

Keywords

  • Cross-link
  • Germinal vesicle breakdown
  • Lysyl oxidase
  • Progesterone
  • Ras
  • Xenopus laevis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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