Microarray analysis identifies a common set of cellular genes modulated by different HCV replicon clones

Anna Rita Ciccaglione, Cinzia Marcantonio, Elena Tritarelli, Paola Tataseo, Alessandro Ferraris, Roberto Bruni, Bruno Dallapiccola, Germano Gerosolimo, Angela Costantino, Maria Rapicetta

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Abstract

Background: Hepatitis C virus (HCV) RNA synthesis and protein expression affect cell homeostasis by modulation of gene expression. The impact of HCV replication on global cell transcription has not been fully evaluated. Thus, we analysed the expression profiles of different clones of human hepatoma-derived Huh-7 cells carrying a self-replicating HCV RNA which express all viral proteins (HCV replicon system). Results: First, we compared the expression profile of HCV replicon clone 21-5 with both the Huh-7 parental cells and the 21-5 cured (21-5c) cells. In these latter, the HCV RNA has been eliminated by IFN-α treatment. To confirm data, we also analyzed microarray results from both the 21-5 and two other HCV replicon clones, 22-6 and 21-7, compared to the Huh-7 cells. The study was carried out by using the Applied Biosystems (AB) Human Genome Survey Microarray v1.0 which provides 31,700 probes that correspond to 27,868 human genes. Microarray analysis revealed a specific transcriptional program induced by HCV in replicon cells respect to both IFN-α-cured and Huh-7 cells. From the original datasets of differentially expressed genes, we selected by Venn diagrams a final list of 38 genes modulated by HCV in all clones. Most of the 38 genes have never been described before and showed high fold-change associated with significant p-value, strongly supporting data reliability. Classification of the 38 genes by Panther System identified functional categories that were significantly enriched in this gene set, such as histones and ribosomal proteins as well as extracellular matrix and intracellular protein traffic. The dataset also included new genes involved in lipid metabolism, extracellular matrix and cytoskeletal network, which may be critical for HCV replication and pathogenesis. Conclusion: Our data provide a comprehensive analysis of alterations in gene expression induced by HCV replication and reveal modulation of new genes potentially useful for selection of antiviral targets.

Original languageEnglish
Article number309
JournalBMC Genomics
Volume9
DOIs
Publication statusPublished - Jun 30 2008

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Replicon
Microarray Analysis
Hepacivirus
Clone Cells
Genes
Virus Replication
RNA
Gene Expression
Ribosomal Proteins
Extracellular Matrix Proteins
Viral Proteins
Human Genome
Protein C
Lipid Metabolism
Histones
Antiviral Agents
Extracellular Matrix
Hepatocellular Carcinoma
Homeostasis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ciccaglione, A. R., Marcantonio, C., Tritarelli, E., Tataseo, P., Ferraris, A., Bruni, R., ... Rapicetta, M. (2008). Microarray analysis identifies a common set of cellular genes modulated by different HCV replicon clones. BMC Genomics, 9, [309]. https://doi.org/10.1186/1471-2164-9-309

Microarray analysis identifies a common set of cellular genes modulated by different HCV replicon clones. / Ciccaglione, Anna Rita; Marcantonio, Cinzia; Tritarelli, Elena; Tataseo, Paola; Ferraris, Alessandro; Bruni, Roberto; Dallapiccola, Bruno; Gerosolimo, Germano; Costantino, Angela; Rapicetta, Maria.

In: BMC Genomics, Vol. 9, 309, 30.06.2008.

Research output: Contribution to journalArticle

Ciccaglione, AR, Marcantonio, C, Tritarelli, E, Tataseo, P, Ferraris, A, Bruni, R, Dallapiccola, B, Gerosolimo, G, Costantino, A & Rapicetta, M 2008, 'Microarray analysis identifies a common set of cellular genes modulated by different HCV replicon clones', BMC Genomics, vol. 9, 309. https://doi.org/10.1186/1471-2164-9-309
Ciccaglione AR, Marcantonio C, Tritarelli E, Tataseo P, Ferraris A, Bruni R et al. Microarray analysis identifies a common set of cellular genes modulated by different HCV replicon clones. BMC Genomics. 2008 Jun 30;9. 309. https://doi.org/10.1186/1471-2164-9-309
Ciccaglione, Anna Rita ; Marcantonio, Cinzia ; Tritarelli, Elena ; Tataseo, Paola ; Ferraris, Alessandro ; Bruni, Roberto ; Dallapiccola, Bruno ; Gerosolimo, Germano ; Costantino, Angela ; Rapicetta, Maria. / Microarray analysis identifies a common set of cellular genes modulated by different HCV replicon clones. In: BMC Genomics. 2008 ; Vol. 9.
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