Abstract
Though extensive studies have been conducted, questions regarding the molecular effectors and pathways underlying the regulatory role of 1,25(OH) 2D 3 in human osteoblasts other than cell differentiation and matrix protein production remain unanswered. This study aims to identify genes and pathways that are modulated by 1,25(OH) 2D 3 treatment in human osteoblasts. Primary osteoblast cultures obtained from human bone tissue samples were treated with 1,25(OH) 2D 3 (10 -7 M) for 24 h and their transcritptomes were profiled by microarray analysis using the Affymetrix GeneChip®. Statistical analysis was conducted to identify genes whose expression is significantly modulated following 1,25(OH) 2D 3 treatment. One hundred and fifty-eight genes were found to be differentially expressed. Of these, 136 were upregulated, indicating clear transcriptional activation by 1,25(OH) 2D 3. Biostatistical evaluation of microarray data by Ingenuity Pathways Analysis (IPA) revealed a relevant modulation of genes involved in vitamin D metabolism (CYP24), immune functions (CD14), neurotransmitter transporters (SLC1A1, SLC22A3), and coagulation [thrombomodulin (THBD), tissue plasminogen activator (PLAT), endothelial protein C receptor (PROCR), thrombin receptor (F2R)]. We identified a restricted number of highly regulated genes and confirmed their differential expression by real-time quantitative PCR (RT qPCR). The present genome-wide microarray analysis on 1,25(OH) 2D 3-treated human osteoblasts reveals an interplay of critical regulatory and metabolic pathways and supports the hypothesis that 1,25(OH) 2D 3 can modulate the coagulation process through osteoblasts, activates osteoclastogenesis through inflammation signaling, modulates the effects of monoamines by affecting their reuptake.
| Original language | English |
|---|---|
| Pages (from-to) | 640-649 |
| Number of pages | 10 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 113 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2012 |
Fingerprint
Keywords
- 1,25(OH) D
- Human primary osteoblasts
- Microarray
- Transcriptional profiling
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
Cite this
Microarray analysis of 1,25(OH) 2D 3 regulated gene expression in human primary osteoblasts. / Tarroni, Paola; Villa, Isabella; Mrak, Emanuela; Zolezzi, Francesca; Mattioli, Michela; Gattuso, Claudio; Rubinacci, Alessandro.
In: Journal of Cellular Biochemistry, Vol. 113, No. 2, 02.2012, p. 640-649.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microarray analysis of 1,25(OH) 2D 3 regulated gene expression in human primary osteoblasts
AU - Tarroni, Paola
AU - Villa, Isabella
AU - Mrak, Emanuela
AU - Zolezzi, Francesca
AU - Mattioli, Michela
AU - Gattuso, Claudio
AU - Rubinacci, Alessandro
PY - 2012/2
Y1 - 2012/2
N2 - Though extensive studies have been conducted, questions regarding the molecular effectors and pathways underlying the regulatory role of 1,25(OH) 2D 3 in human osteoblasts other than cell differentiation and matrix protein production remain unanswered. This study aims to identify genes and pathways that are modulated by 1,25(OH) 2D 3 treatment in human osteoblasts. Primary osteoblast cultures obtained from human bone tissue samples were treated with 1,25(OH) 2D 3 (10 -7 M) for 24 h and their transcritptomes were profiled by microarray analysis using the Affymetrix GeneChip®. Statistical analysis was conducted to identify genes whose expression is significantly modulated following 1,25(OH) 2D 3 treatment. One hundred and fifty-eight genes were found to be differentially expressed. Of these, 136 were upregulated, indicating clear transcriptional activation by 1,25(OH) 2D 3. Biostatistical evaluation of microarray data by Ingenuity Pathways Analysis (IPA) revealed a relevant modulation of genes involved in vitamin D metabolism (CYP24), immune functions (CD14), neurotransmitter transporters (SLC1A1, SLC22A3), and coagulation [thrombomodulin (THBD), tissue plasminogen activator (PLAT), endothelial protein C receptor (PROCR), thrombin receptor (F2R)]. We identified a restricted number of highly regulated genes and confirmed their differential expression by real-time quantitative PCR (RT qPCR). The present genome-wide microarray analysis on 1,25(OH) 2D 3-treated human osteoblasts reveals an interplay of critical regulatory and metabolic pathways and supports the hypothesis that 1,25(OH) 2D 3 can modulate the coagulation process through osteoblasts, activates osteoclastogenesis through inflammation signaling, modulates the effects of monoamines by affecting their reuptake.
AB - Though extensive studies have been conducted, questions regarding the molecular effectors and pathways underlying the regulatory role of 1,25(OH) 2D 3 in human osteoblasts other than cell differentiation and matrix protein production remain unanswered. This study aims to identify genes and pathways that are modulated by 1,25(OH) 2D 3 treatment in human osteoblasts. Primary osteoblast cultures obtained from human bone tissue samples were treated with 1,25(OH) 2D 3 (10 -7 M) for 24 h and their transcritptomes were profiled by microarray analysis using the Affymetrix GeneChip®. Statistical analysis was conducted to identify genes whose expression is significantly modulated following 1,25(OH) 2D 3 treatment. One hundred and fifty-eight genes were found to be differentially expressed. Of these, 136 were upregulated, indicating clear transcriptional activation by 1,25(OH) 2D 3. Biostatistical evaluation of microarray data by Ingenuity Pathways Analysis (IPA) revealed a relevant modulation of genes involved in vitamin D metabolism (CYP24), immune functions (CD14), neurotransmitter transporters (SLC1A1, SLC22A3), and coagulation [thrombomodulin (THBD), tissue plasminogen activator (PLAT), endothelial protein C receptor (PROCR), thrombin receptor (F2R)]. We identified a restricted number of highly regulated genes and confirmed their differential expression by real-time quantitative PCR (RT qPCR). The present genome-wide microarray analysis on 1,25(OH) 2D 3-treated human osteoblasts reveals an interplay of critical regulatory and metabolic pathways and supports the hypothesis that 1,25(OH) 2D 3 can modulate the coagulation process through osteoblasts, activates osteoclastogenesis through inflammation signaling, modulates the effects of monoamines by affecting their reuptake.
KW - 1,25(OH) D
KW - Human primary osteoblasts
KW - Microarray
KW - Transcriptional profiling
UR - http://www.scopus.com/inward/record.url?scp=84855533226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855533226&partnerID=8YFLogxK
U2 - 10.1002/jcb.23392
DO - 10.1002/jcb.23392
M3 - Article
C2 - 21956231
AN - SCOPUS:84855533226
VL - 113
SP - 640
EP - 649
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 2
ER -