Microarray analysis on human neuroblastoma cells exposed to aluminum, β 1-42-Amyloid or the β1-42-Amyloid aluminum complex

Valentina Gatta, Denise Drago, Karina Fincati, Maria Teresa Valenti, Luca Dalle Carbonare, Stefano L. Sensi, Paolo Zatta

Research output: Contribution to journalArticlepeer-review

Abstract

Background: A typical pathological feature of Alzheimer's disease (AD) is the appearance in the brain of senile plaques made up of β-amyloid (Aβ) and neurofibrillary tangles. AD is also associated with an abnormal accumulation of some metal ions, and we have recently shown that one of these, aluminum (Al), plays a relevant role in affecting Ab aggregation and neurotoxicity. Methodology: In this study, employing a microarray analysis of 35,129 genes, we investigated the effects induced by the exposure to the Aβ 1-42-Al (Aβ-Al) complex on the gene expression profile of the neuronal-like cell line, SH-SY5Y. Principal Findings: The microarray assay indicated that, compared to Aβ or Al alone, exposure to Aβ-Al complex produced selective changes in gene expression. Some of the genes selectively over or underexpressed are directly related to AD. A further evaluation performed with Ingenuity Pathway analysis revealed that these genes are nodes of networks and pathways that are involved in the modulation of Ca2+ homeostasis as well as in the regulation of glutamatergic transmission and synaptic plasticity. Conclusions and Significance: A-Al appears to be largely involved in the molecular machinery that regulates neuronal as well as synaptic dysfunction and loss. Aβ-Al seems critical in modulating key AD-related pathways such as glutamatergic transmission, Ca2+homeostasis, oxidative stress, inflammation, and neuronal apoptosis.

Original languageEnglish
Article numbere15965
JournalPLoS One
Volume6
Issue number1
DOIs
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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