Microarray analysis on human neuroblastoma cells exposed to aluminum, β 1-42-Amyloid or the β1-42-Amyloid aluminum complex

Valentina Gatta, Denise Drago, Karina Fincati, Maria Teresa Valenti, Luca Dalle Carbonare, Stefano L. Sensi, Paolo Zatta

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: A typical pathological feature of Alzheimer's disease (AD) is the appearance in the brain of senile plaques made up of β-amyloid (Aβ) and neurofibrillary tangles. AD is also associated with an abnormal accumulation of some metal ions, and we have recently shown that one of these, aluminum (Al), plays a relevant role in affecting Ab aggregation and neurotoxicity. Methodology: In this study, employing a microarray analysis of 35,129 genes, we investigated the effects induced by the exposure to the Aβ 1-42-Al (Aβ-Al) complex on the gene expression profile of the neuronal-like cell line, SH-SY5Y. Principal Findings: The microarray assay indicated that, compared to Aβ or Al alone, exposure to Aβ-Al complex produced selective changes in gene expression. Some of the genes selectively over or underexpressed are directly related to AD. A further evaluation performed with Ingenuity Pathway analysis revealed that these genes are nodes of networks and pathways that are involved in the modulation of Ca2+ homeostasis as well as in the regulation of glutamatergic transmission and synaptic plasticity. Conclusions and Significance: A-Al appears to be largely involved in the molecular machinery that regulates neuronal as well as synaptic dysfunction and loss. Aβ-Al seems critical in modulating key AD-related pathways such as glutamatergic transmission, Ca2+homeostasis, oxidative stress, inflammation, and neuronal apoptosis.

Original languageEnglish
Article numbere15965
JournalPLoS One
Volume6
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

amyloid
Alzheimer disease
Microarray Analysis
Microarrays
Aluminum
Neuroblastoma
Amyloid
aluminum
Alzheimer Disease
Genes
Gene expression
homeostasis
Homeostasis
cells
calcium
gene expression
Neurofibrillary Tangles
Neuronal Plasticity
Oxidative stress
neurotoxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gatta, V., Drago, D., Fincati, K., Valenti, M. T., Carbonare, L. D., Sensi, S. L., & Zatta, P. (2011). Microarray analysis on human neuroblastoma cells exposed to aluminum, β 1-42-Amyloid or the β1-42-Amyloid aluminum complex. PLoS One, 6(1), [e15965]. https://doi.org/10.1371/journal.pone.0015965

Microarray analysis on human neuroblastoma cells exposed to aluminum, β 1-42-Amyloid or the β1-42-Amyloid aluminum complex. / Gatta, Valentina; Drago, Denise; Fincati, Karina; Valenti, Maria Teresa; Carbonare, Luca Dalle; Sensi, Stefano L.; Zatta, Paolo.

In: PLoS One, Vol. 6, No. 1, e15965, 2011.

Research output: Contribution to journalArticle

Gatta, Valentina ; Drago, Denise ; Fincati, Karina ; Valenti, Maria Teresa ; Carbonare, Luca Dalle ; Sensi, Stefano L. ; Zatta, Paolo. / Microarray analysis on human neuroblastoma cells exposed to aluminum, β 1-42-Amyloid or the β1-42-Amyloid aluminum complex. In: PLoS One. 2011 ; Vol. 6, No. 1.
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