Microbial tracking of multidrug-resistant Klebsiella pneumoniae isolates in a pediatric hospital setting

P. Bernaschi, F. Del Chierico, A. Petrucca, A. Argentieri, M. Ciofi Degli Atti, G. Ciliento, M. Carletti, M. Muraca, F. Locatelli, Lorenza Putignani

Research output: Contribution to journalArticlepeer-review


We investigated the clonal relatedness of seven multi-drug-resistant (MDR) Klebsiella pneumoniae isolates, as well as three susceptible K. pneumoniae isolates collected during hospital outbreaks and outbreak-related microbiological surveillance, respectively. The relatedness among K. pneumoniae isolates was assessed by pulsed field gel electrophoresis (PFGE) and automated repetitive-sequence-based PCR (rep-PCR) genotyping and the results were compared to a proteomic phenotyping performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). All typing methods agreed on the generation of three different clusters of K. pneumoniae isogenetic/related MDR strains. After strengthening hospital infection control measures, no other spreading events involving MDR-K. pneumoniae were reported until the end of the observation period. This preliminary investigation suggests that, in a hierarchical approach to bacterial typing, MALDI-TOF MS proteome profiling might offer a fast and valuable preliminary screening tool able to support microbiologists during nosocomial outbreak surveys.

Original languageEnglish
Pages (from-to)463-472
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Issue number2
Publication statusPublished - Apr 2013


  • Klebsiella pneumoniae multi-drug-resistant (MDR) strains
  • MALDI-TOF MS proteome profiling
  • Microbial typing

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy


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