Microbubble-assisted p53, RB, and p130 gene transfer in combination with radiation therapy in prostate cancer

Rounak Nande, Adelaide Greco, Michael S. Gossman, Jeffrey P. Lopez, Luigi Claudio, Marco Salvatore, Arturo Brunetti, James Denvir, Candace M. Howard, Pier Paolo Claudio

Research output: Contribution to journalArticlepeer-review

Abstract

Combining radiation therapy and direct intratumoral (IT) injection of adenoviral vectors has been explored as a means to enhance the therapeutic potential of gene transfer. A major challenge for gene transfer is systemic delivery of nucleic acids directly into an affected tissue. Ultrasound (US) contrast agents (microbubbles) are viable candidates to enhance targeted delivery of systemically administered genes. Here we show that p53, pRB, and p130 gene transfer mediated by US cavitation of microbubbles at the tumor site resulted in targeted gene transduction and increased reduction in tumor growth compared to DU-145 prostate cancer cell xenografts treated intratumorally with adenovirus (Ad) or radiation alone. Microbubble-assisted/US-mediated Ad.p53 and Ad.RB treated tumors showed significant reduction in tumor volume compared to Ad.p130 treated tumors (p

Original languageEnglish
Pages (from-to)163-174
Number of pages12
JournalCurrent Gene Therapy
Volume13
Issue number3
DOIs
Publication statusPublished - 2013

Keywords

  • Apoptosis induction
  • External beam radiation
  • Microbubbles
  • P130
  • P53
  • Prostate cancer
  • Radiation
  • RB
  • Retinoblastoma
  • Systemic targeted viral gene delivery
  • Tumor suppressor gene
  • Ultrasound

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)
  • Drug Discovery

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