Microcomputer experience in analysis of flow cytometric DNA distributions

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The program described analyses DNA histograms obtained in flow cytometry using the Gaussians method. The program is written in BASIC to run on a low-cost microcomputer. It utilizes a simple strategy to obtain good estimates of the parameters required for reducing the problem to a task solvable with linear least-squares methods. Features of the program are flexibility, since it is possible to choose different options for parametrization and spacing of Gaussians, and the fact that the operator is not required to provide interactive inspection or inputting parameter values. The capability and velocity of the program, in all its options, are tested and compared on a series of different (not computer-simulated) histograms obtained in our flow cytometry laboratory. Our results suggest that a fresh approach to parametrization may be useful.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalComputer Programs in Biomedicine
Volume19
Issue number2-3
DOIs
Publication statusPublished - 1985

Fingerprint

Microcomputers
Flow Cytometry
DNA
Least-Squares Analysis
Costs and Cost Analysis

Keywords

  • DNA histograms
  • Flow cytometry
  • Sums of Gaussians

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Microcomputer experience in analysis of flow cytometric DNA distributions. / Ubezio, Paolo.

In: Computer Programs in Biomedicine, Vol. 19, No. 2-3, 1985, p. 159-166.

Research output: Contribution to journalArticle

@article{5d74dfd450094c36860220d54ff1b999,
title = "Microcomputer experience in analysis of flow cytometric DNA distributions",
abstract = "The program described analyses DNA histograms obtained in flow cytometry using the Gaussians method. The program is written in BASIC to run on a low-cost microcomputer. It utilizes a simple strategy to obtain good estimates of the parameters required for reducing the problem to a task solvable with linear least-squares methods. Features of the program are flexibility, since it is possible to choose different options for parametrization and spacing of Gaussians, and the fact that the operator is not required to provide interactive inspection or inputting parameter values. The capability and velocity of the program, in all its options, are tested and compared on a series of different (not computer-simulated) histograms obtained in our flow cytometry laboratory. Our results suggest that a fresh approach to parametrization may be useful.",
keywords = "DNA histograms, Flow cytometry, Sums of Gaussians",
author = "Paolo Ubezio",
year = "1985",
doi = "10.1016/0010-468X(85)90007-8",
language = "English",
volume = "19",
pages = "159--166",
journal = "Computer Programs in Biomedicine",
issn = "0010-468X",
publisher = "Elsevier BV",
number = "2-3",

}

TY - JOUR

T1 - Microcomputer experience in analysis of flow cytometric DNA distributions

AU - Ubezio, Paolo

PY - 1985

Y1 - 1985

N2 - The program described analyses DNA histograms obtained in flow cytometry using the Gaussians method. The program is written in BASIC to run on a low-cost microcomputer. It utilizes a simple strategy to obtain good estimates of the parameters required for reducing the problem to a task solvable with linear least-squares methods. Features of the program are flexibility, since it is possible to choose different options for parametrization and spacing of Gaussians, and the fact that the operator is not required to provide interactive inspection or inputting parameter values. The capability and velocity of the program, in all its options, are tested and compared on a series of different (not computer-simulated) histograms obtained in our flow cytometry laboratory. Our results suggest that a fresh approach to parametrization may be useful.

AB - The program described analyses DNA histograms obtained in flow cytometry using the Gaussians method. The program is written in BASIC to run on a low-cost microcomputer. It utilizes a simple strategy to obtain good estimates of the parameters required for reducing the problem to a task solvable with linear least-squares methods. Features of the program are flexibility, since it is possible to choose different options for parametrization and spacing of Gaussians, and the fact that the operator is not required to provide interactive inspection or inputting parameter values. The capability and velocity of the program, in all its options, are tested and compared on a series of different (not computer-simulated) histograms obtained in our flow cytometry laboratory. Our results suggest that a fresh approach to parametrization may be useful.

KW - DNA histograms

KW - Flow cytometry

KW - Sums of Gaussians

UR - http://www.scopus.com/inward/record.url?scp=0021906736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021906736&partnerID=8YFLogxK

U2 - 10.1016/0010-468X(85)90007-8

DO - 10.1016/0010-468X(85)90007-8

M3 - Article

C2 - 3839734

AN - SCOPUS:0021906736

VL - 19

SP - 159

EP - 166

JO - Computer Programs in Biomedicine

JF - Computer Programs in Biomedicine

SN - 0010-468X

IS - 2-3

ER -