Microdialysis and luminescent probe: analytical and clinical aspects

A. Roda, S. Girotti, B. Grigolo, S. Ghini, G. Carrea, R. Bovara, I. Zini, R. Grimaldi

Research output: Contribution to journalArticlepeer-review

Abstract

Immobilized enzymes are widely used in the clinical laboratory to assay several analytes and enzymes. The use of immobilized enzymes makes these reagents recoverable, disposable and in most cases increases their stability and catalytic activity. In conjunction with bioluminescent enzymes (firefly and bacterial luciferases) and chemiluminescent catalyst (peroxidase) we set up high-sensitive flow sensors based on the use of nylon tube coil or epoxy methacrylate column as solid support. For in-vivo determination a suitable microdialysis probe inserted directly into brain or blood allows continuous measurement of extracellular lactate levels by means of a bioluminescent flow detector system. This procedure performs more measurements in the same time interval than other systems (HPLC), e.g. to give a detailed description of the effects of ischemia, or other pathological events, on lactate concentration in the brain.

Original languageEnglish
Pages (from-to)21-29
Number of pages9
JournalBiosensors and Bioelectronics
Volume6
Issue number1
DOIs
Publication statusPublished - 1991

Keywords

  • ATP
  • Bioluminescence
  • flow sensor
  • immobilized enzymes
  • ischemia
  • lactate
  • methacrylate
  • microdialysis
  • nylon

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

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