Microglial cells respond to amyloidogenic PrP peptide by the production of inflammatory cytokines

Jean Michel Peyrin, Corinne Ida Lasmézas, Stéphane Haïk, Fabrizio Tagliavini, Mario Salmona, Alun Williams, Diane Richie, Jean Philippe Deslys, Dominique Dormont

Research output: Contribution to journalArticlepeer-review

Abstract

The scrapie isoform of the prion protein (PrPres) induces neurodegeneration and gliosis in the central nervous system. These features may be reproduced in vitro on exposure of neuronal and glial cultures to PrPres and the peptide HuPr P106-126. In the present study, we investigated the role of microglial cells and astrocytes in the pathological process by studying their molecular response to PrP 106-126 exposure. PrP 106126 elicited a specific overproduction of pro-inflammatory cytokines IL1β and IL6 in microglial cells (but not increased expression of TNFα, IL10, and TGFβ1) and over-expression of GFAP in astrocytes. These effects were strictly dependent on the ability of the peptide to form amyloid fibrils. These data strongly suggest that microglial cells contribute to prion-related neurodegenerative processes by producing proinflammatory cytokines in the brain areas of amyloid PrP deposition.

Original languageEnglish
Pages (from-to)723-729
Number of pages7
JournalNeuroReport
Volume10
Issue number4
Publication statusPublished - Mar 17 1999

Keywords

  • Astrocytes
  • Microglia
  • Mouse
  • Neurodegeneration
  • Prion protein

ASJC Scopus subject areas

  • Neuroscience(all)

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