Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators

Lorenzo Vannozzi; Leonardo Ricotti; Shaikha Alyassi; Claudia Bearzi; Cesare Gargioli; Roberto Rizzi; Kinda Khalaf; Paolo Dario; Arianna Menciassi

doi:10.1109/EMBC.2015.7318372

Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators

Lorenzo Vannozzi, Leonardo Ricotti, Shaikha Alyassi, Claudia Bearzi, Cesare Gargioli, Roberto Rizzi, Kinda Khalaf, Paolo Dario, Arianna Menciassi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper aims to demonstrate the possibility of exploiting poly-L-lactic acid (PLLA) ultra-thin films as platforms for bio-hybrid actuation. Firstly, flat PLLA nanofilms at different concentrations (15 and 25 mg/ml in dichloromethane) were tested with contractile cardiomyocytes. The results obtained using motion vector analysis, a non-invasive method capable of estimating flow velocities on recorded videos, demonstrated that PLLA nanofilms were able to move under the contraction of muscle cells. Immunofluorescence images reflected good cell spreading, thus confirming that these films are promising matrices for bio-hybrid actuation. Subsequently, microgrooved PLLA nanofilms were fabricated, in order to drive muscle cell distribution on an anisotropic surface, thus optimizing the system's efficiency. After matrix characterization, in terms of AFM and SEM imaging, we investigated the viability and morphology of C2C12 skeletal muscle cells (a more controllable muscle cell type), 24 h after cell seeding as well as at the 7-day differentiation state.

Original language	English
Title of host publication	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	354-357
Number of pages	4
Volume	2015-November
ISBN (Print)	9781424492718
DOIs	https://doi.org/10.1109/EMBC.2015.7318372
Publication status	Published - Nov 4 2015
Event	37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015 - Milan, Italy Duration: Aug 25 2015 → Aug 29 2015

Other

Other	37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
Country/Territory	Italy
City	Milan
Period	8/25/15 → 8/29/15

ASJC Scopus subject areas

Computer Vision and Pattern Recognition
Signal Processing
Biomedical Engineering
Health Informatics

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10.1109/EMBC.2015.7318372

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Vannozzi, L., Ricotti, L., Alyassi, S., Bearzi, C., Gargioli, C., Rizzi, R., Khalaf, K., Dario, P., & Menciassi, A. (2015). Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (Vol. 2015-November, pp. 354-357). [7318372] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2015.7318372

Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators. / Vannozzi, Lorenzo; Ricotti, Leonardo; Alyassi, Shaikha et al.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol. 2015-November Institute of Electrical and Electronics Engineers Inc., 2015. p. 354-357 7318372.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Vannozzi, L, Ricotti, L, Alyassi, S, Bearzi, C, Gargioli, C, Rizzi, R, Khalaf, K, Dario, P & Menciassi, A 2015, Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. vol. 2015-November, 7318372, Institute of Electrical and Electronics Engineers Inc., pp. 354-357, 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015, Milan, Italy, 8/25/15. https://doi.org/10.1109/EMBC.2015.7318372

Vannozzi L, Ricotti L, Alyassi S, Bearzi C, Gargioli C, Rizzi R et al. Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. Vol. 2015-November. Institute of Electrical and Electronics Engineers Inc. 2015. p. 354-357. 7318372 https://doi.org/10.1109/EMBC.2015.7318372

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abstract = "This paper aims to demonstrate the possibility of exploiting poly-L-lactic acid (PLLA) ultra-thin films as platforms for bio-hybrid actuation. Firstly, flat PLLA nanofilms at different concentrations (15 and 25 mg/ml in dichloromethane) were tested with contractile cardiomyocytes. The results obtained using motion vector analysis, a non-invasive method capable of estimating flow velocities on recorded videos, demonstrated that PLLA nanofilms were able to move under the contraction of muscle cells. Immunofluorescence images reflected good cell spreading, thus confirming that these films are promising matrices for bio-hybrid actuation. Subsequently, microgrooved PLLA nanofilms were fabricated, in order to drive muscle cell distribution on an anisotropic surface, thus optimizing the system's efficiency. After matrix characterization, in terms of AFM and SEM imaging, we investigated the viability and morphology of C2C12 skeletal muscle cells (a more controllable muscle cell type), 24 h after cell seeding as well as at the 7-day differentiation state.",

author = "Lorenzo Vannozzi and Leonardo Ricotti and Shaikha Alyassi and Claudia Bearzi and Cesare Gargioli and Roberto Rizzi and Kinda Khalaf and Paolo Dario and Arianna Menciassi",

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AU - Alyassi, Shaikha

AU - Bearzi, Claudia

AU - Gargioli, Cesare

AU - Rizzi, Roberto

AU - Khalaf, Kinda

AU - Dario, Paolo

AU - Menciassi, Arianna

PY - 2015/11/4

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N2 - This paper aims to demonstrate the possibility of exploiting poly-L-lactic acid (PLLA) ultra-thin films as platforms for bio-hybrid actuation. Firstly, flat PLLA nanofilms at different concentrations (15 and 25 mg/ml in dichloromethane) were tested with contractile cardiomyocytes. The results obtained using motion vector analysis, a non-invasive method capable of estimating flow velocities on recorded videos, demonstrated that PLLA nanofilms were able to move under the contraction of muscle cells. Immunofluorescence images reflected good cell spreading, thus confirming that these films are promising matrices for bio-hybrid actuation. Subsequently, microgrooved PLLA nanofilms were fabricated, in order to drive muscle cell distribution on an anisotropic surface, thus optimizing the system's efficiency. After matrix characterization, in terms of AFM and SEM imaging, we investigated the viability and morphology of C2C12 skeletal muscle cells (a more controllable muscle cell type), 24 h after cell seeding as well as at the 7-day differentiation state.

AB - This paper aims to demonstrate the possibility of exploiting poly-L-lactic acid (PLLA) ultra-thin films as platforms for bio-hybrid actuation. Firstly, flat PLLA nanofilms at different concentrations (15 and 25 mg/ml in dichloromethane) were tested with contractile cardiomyocytes. The results obtained using motion vector analysis, a non-invasive method capable of estimating flow velocities on recorded videos, demonstrated that PLLA nanofilms were able to move under the contraction of muscle cells. Immunofluorescence images reflected good cell spreading, thus confirming that these films are promising matrices for bio-hybrid actuation. Subsequently, microgrooved PLLA nanofilms were fabricated, in order to drive muscle cell distribution on an anisotropic surface, thus optimizing the system's efficiency. After matrix characterization, in terms of AFM and SEM imaging, we investigated the viability and morphology of C2C12 skeletal muscle cells (a more controllable muscle cell type), 24 h after cell seeding as well as at the 7-day differentiation state.

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Microgrooved ultra-thin films as building blocks of future bio-hybrid actuators

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