Microheterogeneity of serum glycosyl albumin in diabetes mellitus

G. M. Ghiggeri, G. Candiano, G. Delfino, G. Pallavicini, C. Queirolo

Research output: Contribution to journalArticlepeer-review


Proteins purified by affinity chromatography on Blue-Sepharose CL-6B from serum of 10 normal controls and 19 Type I diabetic patients were studied by means of combined ultrathin isoelectric focusing and photochemical silver stain. While only a single band of protein (characterized as albumin by crossed immunoelectrophoresis) with a pI of 4.7 was found in serum of normal subjects, 10 out of the diabetic group showed some bands of proteins with a pI greater than 4.7 and a single one with a pI less than 4.7. Concanavalin A-Sepharose removed all these bands with altered pIs which were further characterized as albumin by 1) fused rocket immunoelectrophoresis of the elutates from Concanavalin A-Sepharose, 2) direct immunofixation after isoelectric focusing of proteins with high purified anti-albumin antibodies, 3) SDS-polyacrylamide greadient pore electrophoresis, 4) aminoacidic analysis. The gaschromatographic analysis of carbohydrates released from both the albumin bound to Concanavalin A-Sepharose and that not bound, revealed in addition to two unidentified peaks, the presence of glucose, galactose and mannose whose contents were greatly increased in albumin with affinity for lectin. Serum glycosyl albumin concentration was not statistically different in serum of diabetic patients displaying cationic glycosyl albumin in comparison to patients without these proteins (0.2261 ± 0.0186 versus 0.1874 ± 0.015 nmole HMF/nmole albumin), whereas the first group showed statistically higher urinary excretion rates of albumin (28.6 ± 1.2 μg/min versus 4.6 ± 0.2 μg/min). These results provide evidence for a variety of glycosyl albumin with a high content of carbohydrates and altered pIs which may have a pathogenetic role in diabetic nephropathy.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalDiabete et Metabolisme
Issue number3
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism


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