Micronucleus assay in human cells: Lymphocytes and buccal cells

Claudia Bolognesi, Michael Fenech

Research output: Chapter in Book/Report/Conference proceedingChapter

24 Citations (Scopus)

Abstract

The micronucleus (MN) assay, applied in different surrogate tissues, is one of the best validated cytogenetic techniques for evaluating chromosomal damage in humans. The cytokinesis-block micronucleus cytome assay (CBMNcyt) in peripheral blood lymphocytes is the most frequent method in biomonitoring human populations to evaluate exposure to genotoxic agents, micronutrient deficiency, or excess and genetic instability. Furthermore recent scientific evidence suggests an association between an increased MN frequency in lymphocytes and risk of cancer and other age-related degenerative diseases. The micronucleus cytome assay applied in buccal exfoliated cells (BMNCyt) provides a complementary method for measuring DNA damage and cytotoxic effects in an easily accessible tissue not requiring in vitro culture. The protocol for CBMNcyt described here refers to the use of ex vivo whole blood involving 72 h of culture with the block of cytokinesis at 44 h. BMNCyt protocol reports the established method for sample processing, slide preparation, and scoring.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages191-207
Number of pages17
Volume1044
ISBN (Print)9781627035286
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1044
ISSN (Print)10643745

Fingerprint

Micronucleus Tests
Cheek
Cytokinesis
Lymphocytes
Environmental Monitoring
Micronutrients
Cytogenetic Analysis
DNA Damage
Population
Neoplasms

Keywords

  • BMCyt
  • Buccal cell
  • Bud
  • CBMNcyt
  • Chromosomal damage
  • Cytome
  • DNA damage
  • Lymphocyte
  • Micronucleus
  • Nucleoplasmic bridge

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bolognesi, C., & Fenech, M. (2013). Micronucleus assay in human cells: Lymphocytes and buccal cells. In Methods in Molecular Biology (Vol. 1044, pp. 191-207). (Methods in Molecular Biology; Vol. 1044). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-529-3_10

Micronucleus assay in human cells : Lymphocytes and buccal cells. / Bolognesi, Claudia; Fenech, Michael.

Methods in Molecular Biology. Vol. 1044 Humana Press Inc., 2013. p. 191-207 (Methods in Molecular Biology; Vol. 1044).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bolognesi, C & Fenech, M 2013, Micronucleus assay in human cells: Lymphocytes and buccal cells. in Methods in Molecular Biology. vol. 1044, Methods in Molecular Biology, vol. 1044, Humana Press Inc., pp. 191-207. https://doi.org/10.1007/978-1-62703-529-3_10
Bolognesi C, Fenech M. Micronucleus assay in human cells: Lymphocytes and buccal cells. In Methods in Molecular Biology. Vol. 1044. Humana Press Inc. 2013. p. 191-207. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-529-3_10
Bolognesi, Claudia ; Fenech, Michael. / Micronucleus assay in human cells : Lymphocytes and buccal cells. Methods in Molecular Biology. Vol. 1044 Humana Press Inc., 2013. pp. 191-207 (Methods in Molecular Biology).
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