Micropatterned geometry shape oligodendrocyte and microglia plasticity

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cellular adhesion is essential in maintaining multicellular structure by connecting cells to each other in vivo and to a biomimetic substrate in vitro. In this context, one of the first steps toward the comprehension, for instance, of oligodendrocyte and microglia adhesion and migrating behavior consists in discriminating the different morphological features that can be acquired when cells are cultured on diverse surface topographies that mimic an in vivo three-dimensional environment. With this in mind, in this chapter, we describe how to exploit the silicon isotropic topography consisting of line-grating geometries and micropillar structures fabricated on polydimethylsiloxane by soft lithography. By reproducing a specialized niche for the cells, micropatterned biomimetic substrates can help to understand the role of structural determinants in priming morphogenesis of oligodendrocytes and microglia and can be exploited for translational research on functional tissue engineering and implantable device design.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-154
Number of pages16
Volume1727
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1727
ISSN (Print)1064-3745

Fingerprint

Biomimetics
Oligodendroglia
Microglia
Equipment Design
Translational Medical Research
Silicon
Tissue Engineering
Morphogenesis
Cultured Cells
baysilon
In Vitro Techniques

Keywords

  • Adhesion
  • Biomimetic micropatterned substrate
  • Cell morphology
  • Microglia
  • Oligodendrocyte

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Volonté, C., De Ninno, A., & Amadio, S. (2018). Micropatterned geometry shape oligodendrocyte and microglia plasticity. In Methods in Molecular Biology (Vol. 1727, pp. 139-154). (Methods in Molecular Biology; Vol. 1727). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7571-6_11

Micropatterned geometry shape oligodendrocyte and microglia plasticity. / Volonté, Cinzia; De Ninno, Adele; Amadio, Susanna.

Methods in Molecular Biology. Vol. 1727 Humana Press Inc., 2018. p. 139-154 (Methods in Molecular Biology; Vol. 1727).

Research output: Chapter in Book/Report/Conference proceedingChapter

Volonté, C, De Ninno, A & Amadio, S 2018, Micropatterned geometry shape oligodendrocyte and microglia plasticity. in Methods in Molecular Biology. vol. 1727, Methods in Molecular Biology, vol. 1727, Humana Press Inc., pp. 139-154. https://doi.org/10.1007/978-1-4939-7571-6_11
Volonté C, De Ninno A, Amadio S. Micropatterned geometry shape oligodendrocyte and microglia plasticity. In Methods in Molecular Biology. Vol. 1727. Humana Press Inc. 2018. p. 139-154. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7571-6_11
Volonté, Cinzia ; De Ninno, Adele ; Amadio, Susanna. / Micropatterned geometry shape oligodendrocyte and microglia plasticity. Methods in Molecular Biology. Vol. 1727 Humana Press Inc., 2018. pp. 139-154 (Methods in Molecular Biology).
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