Microplate enzyme-linked immunosorbent assay in the study of the structural relationship between myosin light chains

Donatella Biral, Luciano Dalla Libera, Claudio Franceschi, Alfredo Margreth

Research output: Contribution to journalArticle

Abstract

A microplate enzyme-linked immunosorbent assay (microELISA) for the study of immunochemical relationships between rabbit myosin light chains is described. Purified individual fast-muscle myosin light chains (LC1F, LC2F and LC3F) and their respective antisera, obtained in chicken, were used. Optimal conditions for antigen concentration, antiserum dilution, substrate concentration, incubation time and reproducibility with time were established. The observed cross-reactivities between the different types of light chains associated with rabbit fast-muscle myosin confirm and extend previous results obtained by other authors using radioimmunoassay procedures. It was concluded that microELISA may be successfully employed also to the study of macromolecule cross-reactivities.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalJournal of Immunological Methods
Volume31
Issue number1-2
DOIs
Publication statusPublished - Dec 14 1979

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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