Microplate selection technique (MPST). A new method for selecting mouse transfectants expressing human gene products

L. Peruzzi, G. Melioli, L. B. De Monte, M. Alessio, M. Zaccolo, S. Roggero, G. Bellone, A. Funaro, F. Malavasi

Research output: Contribution to journalArticle

Abstract

This paper examines the analytical power of fluorescence activated cell sorting and immunorosetting technique as compared with the newly devised microplate selection technique in identifying transfected murine L cells expressing human surface molecules. The microplate selection technique relies on the mechanical transfer of transfected cells to a terasaki microplate, where an indirect immunofluorescence assay is carried out. It is a simple procedure not requiring costly equipment and with a detection capacity equivalent to that of the fluorescence activated cell sorter. The microplate selection technique proved to be sensitive enough to detect all the transfectants produced during the present study.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalJournal of Immunological Methods
Volume123
Issue number1
DOIs
Publication statusPublished - Sep 29 1989

Keywords

  • Fluorescence activated cell sorter
  • Immunorosette
  • Monoclonal antibody
  • Surface molecule
  • Transfectants murine

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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