MicroRNA-155 in serum-derived extracellular vesicles as a potential biomarker for hematologic malignancies - a short report

Antonella Caivano, Francesco la Rocca, Vittorio Simeon, Marco Girasole, Simone Dinarelli, Ilaria Laurenzana, Angelo de Stradis, Luciana de Luca, Stefania Trino, Antonio Traficante, Giovanni D’Arena, Giovanna Mansueto, Oreste Villani, Giuseppe Pietrantuono, Luca Laurenti, Luigi Del Vecchio, Pellegrino Musto

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The use of extracellular vesicles (EVs) from body fluids as “liquid biopsies” is emerging as a promising approach for the diagnosis, prognosis and therapeutic monitoring of cancer patients. MicroRNA-155 (miR155), a non-coding transcript of the B-cell integration cluster (BIC) gene, has been reported to play a critical role in the pathogenesis of several types of hematologic malignancies (HMs) in which high miR155 levels have been found. At yet, however, the EV miR155 level and its putative clinical relevance in sera of HM patients have not been reported. Methods: EVs from sera of representative patients with eight different HMs and healthy subjects (controls) were isolated using differential centrifugation. The identity and quality of the EVs were verified by atomic force and transmission electron microscopy. The EV miR155 levels were measured by quantitative RT-PCR. The sensitivity, specificity and area under the curve (AUC) of differences in EV miR155 levels were determined using ROC curve analyses. Results: We found that the EV miR155 levels were significantly higher in chronic lymphocytic leukemia (CLL), acute myeloid leukemia (AML) and Waldenström’s macroglobulinemia (WM) cases compared to controls. Conversely, we found that the EV miR155 levels were significantly lower in myelodysplastic syndrome (MDS) and multiple myeloma (MM) cases. No differences were found in follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL) or Hodgkin’s Lymphoma (HL) cases compared to controls. EV miR155 ROC curve analyses revealed significantly different patterns in CLL and AML cases compared to controls, and in AML cases compared to MDS cases (p = 0.004, p = 0.01 and p = 0.04, respectively). In addition, we found that high EV miR155 levels correlated with high white blood cell counts in AML patients. Conclusion: Our data indicate that EV miR155 may serve as an attractive new, non-invasive diagnostic biomarker in human hematologic malignancies.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalCellular Oncology
DOIs
Publication statusAccepted/In press - Oct 19 2016

Keywords

  • Acute myeloid leukemia
  • Chronic lymphocytic leukemia
  • Extracellular vesicles
  • miR155
  • Myelodysplastic syndrome
  • Waldenström’s macroglobulinemia

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'MicroRNA-155 in serum-derived extracellular vesicles as a potential biomarker for hematologic malignancies - a short report'. Together they form a unique fingerprint.

Cite this