MicroRNA-210 modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand ephrin-A3

Pasquale Fasanaro, Yuri D'Alessandra, Valeria Di Stefano, Roberta Melchionna, Sveva Romani, Giulio Pompilio, Maurizio C. Capogrossi, Fabio Martelli

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are small non-protein-coding RNAs that function as negative gene expression regulators. In the present study, we investigated miRNAs role in endothelial cell response to hypoxia. We found that the expression of miR-210 progressively increased upon exposure to hypoxia. miR-210 overexpression in normoxic endothelial cells stimulated the formation of capillary-like structures on Matrigel and vascular endothelial growth factor-driven cell migration. Conversely, miR-210 blockade via anti-miRNA transfection inhibited the formation of capillary-like structures stimulated by hypoxia and decreased cell migration in response to vascular endothelial growth factor. miR-210 overexpression did not affect endothelial cell growth in both normoxia and hypoxia. However, anti-miR-210 transfection inhibited cell growth and induced apoptosis, in both normoxia and hypoxia. We determined that one relevant target of miR-210 in hypoxia was Ephrin-A3 since miR-210 was necessary and sufficient to down-modulate its expression. Moreover, luciferase reporter assays showed that Ephrin-A3 was a direct target of miR-210. Ephrin-A3 modulation by miR-210 had significant functional consequences; indeed, the expression of an Ephrin-A3 allele that is not targeted by miR-210 prevented miR-210-mediated stimulation of both tubulogenesis and chemotaxis. We conclude that miR-210 up-regulation is a crucial element of endothelial cell response to hypoxia, affecting cell survival, migration, and differentiation.

Original languageEnglish
Pages (from-to)15878-15883
Number of pages6
JournalJournal of Biological Chemistry
Volume283
Issue number23
DOIs
Publication statusPublished - Jun 6 2008

Fingerprint

Ephrin-A3
Endothelial cells
Receptor Protein-Tyrosine Kinases
MicroRNAs
Endothelial Cells
Ligands
Cell growth
Vascular Endothelial Growth Factor A
Cell Movement
Small Untranslated RNA
Transfection
Luciferases
Gene expression
Assays
Cells
Modulation
Apoptosis
Chemotaxis
Growth
Hypoxia

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

MicroRNA-210 modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand ephrin-A3. / Fasanaro, Pasquale; D'Alessandra, Yuri; Di Stefano, Valeria; Melchionna, Roberta; Romani, Sveva; Pompilio, Giulio; Capogrossi, Maurizio C.; Martelli, Fabio.

In: Journal of Biological Chemistry, Vol. 283, No. 23, 06.06.2008, p. 15878-15883.

Research output: Contribution to journalArticle

Fasanaro, Pasquale ; D'Alessandra, Yuri ; Di Stefano, Valeria ; Melchionna, Roberta ; Romani, Sveva ; Pompilio, Giulio ; Capogrossi, Maurizio C. ; Martelli, Fabio. / MicroRNA-210 modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand ephrin-A3. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 23. pp. 15878-15883.
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