MicroRNA-567 dysregulation contributes to carcinogenesis of breast cancer, targeting tumor cell proliferation, and migration

Gloria Bertoli, Claudia Cava, Cecilia Diceglie, Cristina Martelli, Giampiero Rizzo, Francesca Piccotti, Luisa Ottobrini, Isabella Castiglioni

Research output: Contribution to journalArticle

Abstract

Purpose: We demonstrated that Hsa-miR-567 expression is significantly downregulated in poor prognosis breast cancer, compared to better prognosis breast cancer, having a role in the control of cell proliferation and migration by regulating KPNA4 gene. Methods and results: In this study, based on our previously published in silico results, we proved both in vitro (cell line studies) and ex vivo (clinical studies), that Hsa-miR-567 expression is significantly downregulated in breast cancer with poor prognosis when compared to breast cancer with better prognosis. More intriguingly, we demonstrated that the ectopic expression of Hsa-miR-567 in poor prognosis breast cancer cell line strongly inhibits in vitro cell proliferation and migration. Furthermore, we showed in vivo that breast cancer cells, stably expressing Hsa-miR-567, xenografted in mouse, reduce tumor growth ability. Consistently, we found that karyopherin 4 (KPNA4), predicted target gene of Hsa-miR-567 as identified by our in silico analysis, is upregulated in highly aggressive MDA-MB-231 breast cancer cell line and patient tissues with poor prognosis with respect to good prognosis. Conclusions: Our results suggest a potential role of Hsa-miR-567 as a novel prognostic biomarker for BC and as regulator of KPNA4.

Original languageEnglish
Pages (from-to)605-616
Number of pages12
JournalBreast Cancer Research and Treatment
Volume161
Issue number3
DOIs
Publication statusPublished - Feb 1 2017

Keywords

  • Biomarker
  • Breast cancer
  • MicroRNA/miRNA
  • Prognosis
  • Proliferation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'MicroRNA-567 dysregulation contributes to carcinogenesis of breast cancer, targeting tumor cell proliferation, and migration'. Together they form a unique fingerprint.

  • Cite this

    Bertoli, G., Cava, C., Diceglie, C., Martelli, C., Rizzo, G., Piccotti, F., Ottobrini, L., & Castiglioni, I. (2017). MicroRNA-567 dysregulation contributes to carcinogenesis of breast cancer, targeting tumor cell proliferation, and migration. Breast Cancer Research and Treatment, 161(3), 605-616. https://doi.org/10.1007/s10549-016-4079-2