MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro

Research output: Contribution to journalArticle

Abstract

Smooth muscle cell (SMC) plasticity plays an important role during development and in vascular pathologies such as atherosclerosis and restenosis. It was recently shown that down-regulation of microRNA (miR)-143and -145, which are coexpressed from a single promoter, regulates the switch from contractile to synthetic phenotype, allowing SMCs to migrate and proliferate. We show in this study that loss of miR-143/145 in vitro and in vivo results in the formation of podosomes, which are actin-rich membrane protrusions involved in the migration of several cell types, including SMCs. We further show that platelet-derived growth factor (PDGF) mediates podosome formation in SMCs through the regulation of miR-143/145 expression via a pathway involving Src and p53. Moreover, we identify key podosome regulators as targets of miR-143 (PDGF receptor a and protein kinase C. ε) and miR-145 (fascin). Thus, dysregulation of the miR-143 and -145 genes is causally involved in the aberrant SMC plasticity encountered during vascular disease, in part through the up-regulation of an autoregulatory loop that promotes podosome formation.

Original languageEnglish
Pages (from-to)13-22
Number of pages10
JournalJournal of Cell Biology
Volume189
Issue number1
DOIs
Publication statusPublished - Apr 5 2010

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MicroRNAs
Vascular Smooth Muscle
Smooth Muscle Myocytes
Platelet-Derived Growth Factor Receptors
Platelet-Derived Growth Factor
Vascular Diseases
Protein Kinase C
Cell Movement
Blood Vessels
In Vitro Techniques
Podosomes
Actins
Atherosclerosis
Up-Regulation
Down-Regulation
Pathology
Phenotype
Membranes
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro. / Quintavalle, Manuela; Elia, Leonardo; Condorelli, Gianluigi; Courtneidge, Sara A.

In: Journal of Cell Biology, Vol. 189, No. 1, 05.04.2010, p. 13-22.

Research output: Contribution to journalArticle

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