MicroRNA expression in the cervix during pregnancy is associated with length of gestation

Alison P. Sanders, Heather H. Burris, Allan C. Just, Valeria Motta, Katherine Svensson, Adriana Mercado-Garcia, Ivan Pantic, Joel Schwartz, Martha M. Tellez-Rojo, Robert O. Wright, Andrea A. Baccarelli

Research output: Contribution to journalArticle

Abstract

Preterm birth is a leading cause of infant mortality and can lead to poor life-long health and adverse neurodevelopmental outcomes. The pathophysiologic mechanisms that precede preterm labor remain elusive, and the role that epigenetic phenomena play is largely unstudied. The objective of this study was to assess the association between microRNA (miRNA) expression levels in cervical cells obtained from swabs collected during pregnancy and the length of gestation. We analyzed cervical samples obtained between 16 and 19 weeks of gestation from 53 women in a prospective cohort from Mexico City, and followed them until delivery. Cervical miRNA was extracted and expression was quantified using the NanoString nCounter Analysis System. Linear regression models were used to examine the association between miRNA expression levels and gestational age at delivery, adjusted for maternal age, education, parity, body mass index, smoke exposure, and inflammation assessed on a Papanicolaou smear. We identified 6 miRNAs that were significantly associated with gestational age at the time of delivery, including miR-21, 30e, 142, 148b, 29b, and 223. Notably, per each doubling in miR-21 expression, gestations were 0.9 (95% CI: 0.2–1.5) days shorter on average (P = 0.009). Per each doubling in miR-30e, 142, 148b, 29b, and 223 expression, gestations were shorter by 1.0 to 1.6 days. The predicted targets of the miRNAs were enriched for molecules involved in DNA replication and inflammatory processes. The levels of specific miRNAs in the human cervix during pregnancy are predictive of gestational age at delivery, and should be validated in future studies as potential biomarkers of preterm birth risk.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalEpigenetics
Volume10
Issue number3
DOIs
Publication statusPublished - Jan 22 2015

Keywords

  • Cervix
  • Delivery
  • Gestational age
  • Labor
  • MicroRNA
  • Pap smear
  • Preterm

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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  • Cite this

    Sanders, A. P., Burris, H. H., Just, A. C., Motta, V., Svensson, K., Mercado-Garcia, A., Pantic, I., Schwartz, J., Tellez-Rojo, M. M., Wright, R. O., & Baccarelli, A. A. (2015). MicroRNA expression in the cervix during pregnancy is associated with length of gestation. Epigenetics, 10(3), 221-228. https://doi.org/10.1080/15592294.2015.1006498